Recombinant Human Nucleoporin NUP35 (NUP35)

Code CSB-YP836735HU
MSDS
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Source Yeast
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Code CSB-EP836735HU
MSDS
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Source E.coli
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Code CSB-EP836735HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836735HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP836735HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUP35
Uniprot No.
Alternative Names
35 kDa nucleoporin; Mitotic phosphoprotein 44; MP 44; MP-44; NP 44; NP44; Nuclear pore complex protein 53kDa; Nuclear pore complex protein Nup 53; Nuclear pore complex protein Nup53; Nuclear pore protein NUP 53; Nuclear pore protein NUP53; Nucleoporin 35kDa; Nucleoporin 53kDa; Nucleoporin Nup 35; Nucleoporin NUP 53; Nucleoporin Nup35; Nucleoporin NUP53; NUP 35; Nup 53; NUP35; NUP53; NUP53_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-326
Target Protein Sequence
MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS PGLGSTPLTS RRQPNISVMQ SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY QVISDRQTPK KDESLVSKAM EYMFGW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
Gene References into Functions
  1. defined regions of Nup53 that bind to neighboring nucleoporins as well as those domains that target Nup53 to the nuclear pore complex. PMID: 18256286
Subcellular Location
Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein.
Protein Families
Nup35 family
Database Links

HGNC: 29797

OMIM: 608140

KEGG: hsa:129401

STRING: 9606.ENSP00000295119

UniGene: Hs.180591

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