Recombinant Human P2X purinoceptor 3 (P2RX3), partial

Code CSB-YP017321HU
MSDS
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Source Yeast
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Code CSB-EP017321HU
MSDS
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Source E.coli
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Code CSB-EP017321HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017321HU
MSDS
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Source Baculovirus
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Code CSB-MP017321HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
P2RX3
Uniprot No.
Alternative Names
ATP receptor; MGC129956; P2rx3; P2RX3_HUMAN; P2X purinoceptor 3; P2X receptor subunit 3; P2X3; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 3; Purinergic receptor P2X3; Purinoceptor P2X3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Receptor for ATP that acts as a ligand-gated cation channel. Plays a role in sensory perception. Required for normal perception of pain. Required for normal taste perception.
Gene References into Functions
  1. Druggable negative allosteric site of P2X3 receptors. PMID: 29674445
  2. conclude that the inter-subunit salt bridge between E112 and R198 of the head and dorsal fin domains, respectively, serves to control the mobility of these domains during agonist-activation of the hP2X3R PMID: 26825305
  3. P2X3 expressions in endometriosis endometrium and endometriotic lesions were significantly higher as compared with control endometrium, and positively correlated with pain. PMID: 28898282
  4. P2X3 receptor expression was up-regulated in neurons from patients with temporal lobe epilepsy. PMID: 26738991
  5. X-ray crystal structures of the human P2X3 receptor in apo/resting, agonist-bound/open-pore, agonist-bound/closed-pore/desensitized and antagonist-bound/closed states PMID: 27626375
  6. there is a relationship between the elevated expression of P2X3 receptor and P2X7 receptor in peripheral blood leukocytes and high serum epinephrine and norepinephrine levels in hyperthyroidism patients. PMID: 27312548
  7. Data show that monoclonal antibodies directed against human P2X3 (12D4) potentiated the slow inactivating current mediated by the heteromeric purinergic receptor hP2X2/3 channel. PMID: 27129281
  8. Data indicate P2X3 purinergic receptors as potential new targets for hepatocellular carcinoma (HCC) therapy. PMID: 26517690
  9. Results demonstrate that the stoichiometry of the heterotrimeric hP2X2/3 receptor is not fixed, but determined by the relative availability of P2X2 and P2X3 subunits PMID: 26184350
  10. Urothelial P2X3 receptors decreased significantly in responders after Lipotoxin instillation, but not after BoNT-A injection. PMID: 26241848
  11. The results from this study demonstrate that there is a significant difference in the expression of the purinergic P2X2, P2X3 and P2X7 receptors in the different histological layers of the human urinary bladder. PMID: 26253104
  12. sensory proteins P2X3 and TRPV1 are in correlation with urothelial differentiation, while P2X5 and TRPV4 have unique expression patterns PMID: 24868547
  13. esophagitis increases mRNA expressions of P2X3 and P2X7 receptors accompanied by up-regulation of TRPV1 and neurotrophic factors (NGF and GDNF). PMID: 25336328
  14. Conformational flexibility of the agonist binding jaw of the human P2X3 receptor is a prerequisite for channel opening PMID: 24989924
  15. TRPV1-, TRPV2-, P2X3-, and parvalbumin-immunoreactivity neurons in the human nodose ganglion innervate the pharynx and epiglottis through the pharyngeal branch and superior laryngeal nerve PMID: 24764033
  16. Data indicate that Markov models are suitable to simulate agonist antagonist interactions at fast desensitizing receptors such as the P2X3R. PMID: 24223907
  17. ATP-gated P2X3 channels in enterochromaffin cells are downregulated in ulcerative colitis. PMID: 23917247
  18. An early and broad expression of P2X3 receptors was found in prenatal dorsal root ganglion neurons. PMID: 22052556
  19. HEK 293 cells are not permissive for stable P2X3 expression afer a significant time-dependent cell loss; often used in a recmbinant expression system, HEK cells represent a limiting permissive environment for P2X3 receptors. PMID: 22160848
  20. Structure and function of P2RX3 is reviewed, and its role in treatment of pain is discussed. [Review Article] PMID: 22963434
  21. The effects of single alanine substitutions of amino acid residues in the supposed ATP binding site of the human P2X3 receptor on the agonistic effect of nucleotide analogs. PMID: 22498660
  22. one subunit of P2X2 and two subunits of P2X3 form P2X2/3 heteromeric receptors, whereas two subunits of P2X2 and one subunit of P2X6 constitute P2X2/6 receptors PMID: 22378790
  23. Amino acid residues constituting the agonist binding site of the human P2X3 receptor. PMID: 21098022
  24. P2X3 positive fibres in the trigeminal sensory system were significantly increased in burning mouth syndrome patients compared with controls. PMID: 20418063
  25. The present data indicate a novel target, P2X3 receptors, to express the action of Cdk5 on membrane proteins involved in pain perception. PMID: 19960242
  26. Released ATP activates P2X(3) receptors in the beta-cell plasma membrane, resulting in increased [Ca(2+)](i) and enhanced insulin secretion. PMID: 20308565
  27. P2X(1) receptors showed an anti-inflammatory effect reducing NF-kappaB activation and TNF-alpha release whilst P2X(3) receptors mediated opposite response. PMID: 20110693
  28. Review. Activation of P2X(3)-containing channels is an important mediator of persistent nociceptive signalling. The data also indicate potential discrete roles for homomeric P2X(3) & heteromeric P2X(2/3) receptor activation in acute & chronic pain. PMID: 12885270
  29. in fibers of human tooth pulp ... may play a role in perception of dental pain PMID: 14520770
  30. Effect of acute hypoxia on cloned homo- and heteromeric P2X3 receptors expressed in human embryonic kidney 293 cells. PMID: 15331767
  31. negative residues in the P2X3 ATP receptor ectodomain are structural determinants for desensitization and the Ca2+-sensing modulatory sites PMID: 15475563
  32. These data indicate that PKC activation can enhance both the Ca(2+) signal as well as the cation current through P2X(3)R, however it appears that the regulation is unlikely to be a result of direct phosphorylation of the receptor. PMID: 17052768
  33. genetic and pharmacological approaches have provided converging evidence that activation of P2X3-containing channels is an important mediator of acute and persistent nociceptive signaling--review PMID: 18600577
  34. Sodium Ferrulate may inhibit the initiation of pain and primary afferent sensitization mediated by P2X(3) receptor during chronic constriction injury. PMID: 18805451
  35. These cells expressed P2X3 receptors. Suburothelial myofibroblasts may be intermediate in processing adenosine triphosphate-mediated sensory activation PMID: 19456993

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
P2X receptor family
Database Links

HGNC: 8534

OMIM: 600843

KEGG: hsa:5024

STRING: 9606.ENSP00000263314

UniGene: Hs.146738

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