Recombinant Human PHD finger protein 10 (PHF10)

Code CSB-YP017898HU
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Source Yeast
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Code CSB-EP017898HU
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Source E.coli
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Code CSB-EP017898HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017898HU
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Source Baculovirus
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Code CSB-MP017898HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PHF10
Uniprot No.
Alternative Names
BAF45a; BRG1-associated factor 45a; FLJ10975; MGC111009; PHD finger protein 10; PHD finger protein 10; isoform a; PHD zinc finger protein XAP135; Phf10; PHF10_HUMAN; XAP135
Species
Homo sapiens (Human)
Expression Region
2-498
Target Protein Sequence
AAAAGPGAA LSPRPCDSDP ATPGAQSPKD DNEDNSNDGT QPSKRRRMGS GDSSRSCETS SQDLGFSYYP AENLIEYKWP PDETGEYYML QEQVSEYLGV TSFKRKYPDL ERRDLSHKEK LYLRELNVIT ETQCTLGLTA LRSDEVIDLM IKEYPAKHAE YSVILQEKER QRITDHYKEY SQMQQQNTQK VEASKVPEYI KKAAKKAAEF NSNLNRERME ERRAYFDLQT HVIQVPQGKY KVLPTERTKV SSYPVALIPG QFQEYYKRYS PDELRYLPLN TALYEPPLDP ELPALDSDGD SDDGEDGRGD EKRKNKGTSD SSSGNVSEGE SPPDSQEDSF QGRQKSKDKA ATPRKDGPKR SVLSKSVPGY KPKVIPNAIC GICLKGKESN KKGKAESLIH CSQCENSGHP SCLDMTMELV SMIKTYPWQC MECKTCIICG QPHHEEEMMF CDMCDRGYHT FCVGLGAIPS GRWICDCCQR APPTPRKVGR RGKNSKEG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Gene References into Functions
  1. We studied the expression of S100A4 and the total PHF10 protein in some human cancer cell lines. We have found that, in the cell lines studied, the PHF10 expression is correlated with the S100A4 expression. PMID: 27193724
  2. Phosphorylation of PHF10 isoforms occurs while they are incorporated as a subunit of the PBAF complex, and therefore phosphorylation of PHF10 isoforms may play an essential role in regulation of PBAF complex's function and mechanism of action. PMID: 27239853
  3. The study demonstrates the existence of functionally different PBAF complexes in mammalian cell. It also provides an insight into the molecular structure and role of human PHF10/BAF45a and characterizes it as an essential PBAF subunit. PMID: 24763304
  4. PHF10 repressed caspase-3 expression and impaired the programmed cell death pathway in human gastric cancer at the transcriptional level. PMID: 20530714
  5. Data show that that PHF10 is required for cell growth. PMID: 20068294

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Subcellular Location
Nucleus.
Protein Families
SAYP family
Database Links

HGNC: 18250

OMIM: 613069

KEGG: hsa:55274

STRING: 9606.ENSP00000341805

UniGene: Hs.435933

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