Recombinant Human Paired box protein Pax-7 (PAX7)

Code CSB-YP017493HU
MSDS
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Source Yeast
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Code CSB-EP017493HU
MSDS
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Source E.coli
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Code CSB-EP017493HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017493HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017493HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAX7
Uniprot No.
Alternative Names
FLJ37460; HUP1; OTTHUMP00000002534 ; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; Pax7; PAX7 transcriptional factor ; PAX7/FKHR fusion gene, included; PAX7_HUMAN; PAX7B; RGD1564360; RMS2
Species
Homo sapiens (Human)
Expression Region
1-520
Target Protein Sequence
MAALPGTVPR MMRPAPGQNY PRTGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPRQVA TPDVEKKIEE YKRENPGMFS WEIRDRLLKD GHCDRSTVPS GLVSSISRVL RIKFGKKEEE DEADKKEDDG EKKAKHSIDG ILGDKGNRLD EGSDVESEPD LPLKRKQRRS RTTFTAEQLE ELEKAFERTH YPDIYTREEL AQRTKLTEAR VQVWFSNRRA RWRKQAGANQ LAAFNHLLPG GFPPTGMPTL PPYQLPDSTY PTTTISQDGG STVHRPQPLP PSTMHQGGLA AAAAAADTSS AYGARHSFSS YSDSFMNPAA PSNHMNPVSN GLSPQVMSIL GNPSAVPPQP QADFSISPLH GGLDSATSIS ASCSQRADSI KPGDSLPTSQ AYCPPTYSTT GYSVDPVAGY QYGQYGQSEC LVPWASPVPI PSPTPRASCL FMESYKVVSG WGMSISQMEK LKSSQMEQFT
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Gene References into Functions
  1. Data suggest that paired box gene 7 protein (PAX7) target gene repression is a hallmark of facioscapulohumeral muscular dystrophy (FSHD) that should be considered in the investigation of FSHD pathology and therapy. PMID: 29255294
  2. Transfection experiments showed that the PAX7 splice-site mutation putatively causes nonsense-mediated mRNA decay affecting onlyPAX7 isoform 3. PMID: 28779497
  3. The DUX4 homeodomains mediate inhibition of myogenesis and are functionally exchangeable with the Pax7 homeodomain. PMID: 28935672
  4. EWSR1 fusion protein is required for PAX7 expression in Ewing sarcoma and identify a candidate EWSR1-FLI1-bound PAX7 enhancer that coincides with both a consensus GGAA repeat-containing binding site and a peak of regulatory H3K27 acetylation. PMID: 28643791
  5. miR-206 acts as a tumor suppressor in fusion-negative RMS at least partially through downregulation of PAX7. PMID: 27277678
  6. PAX7 expression is a useful marker of skeletal muscle differentiation in rhabdomyosarcoma, particularly of the embryonal subtype, with specificity for rhabdomyosarcoma and Ewing sarcoma. PMID: 27526298
  7. NTN1 rs9788972 is identified as a risk locus for nonsyndromic orofacial clefts susceptibility in a northern Chinese population; SNPs in PAX7 were not associated with any increased risk PMID: 28489749
  8. RAGE upregulates myogenin which upregulates MyoD and downregulates PAX7, with consequent inhibition of proliferation and stimulation of differentiation. PMID: 24554430
  9. Pax7 responds to NF-kappaB by impairing the regenerative capacity of myogenic cells in the muscle microenvironment to drive muscle wasting in cancer. PMID: 24084740
  10. High prevalence of 1p36 microdeletions in B-cell non-Hodgkin lymphomas is associated with PAX7 loss. PMID: 23205730
  11. Our study replicated previous GWAS findings for markers in VAX1 in the Asian population, and identified rare variants in PAX7 and VAX1 that may contribute to the etiology of CL(P) PMID: 23463464
  12. In addition, eight genes classified as 'second tier' hits in the original study (PAX7, THADA, COL8A1/FILIP1L, DCAF4L2, GADD45G, NTN1, RBFOX3 and FOXE1) showed evidence of linkage and association in this replication sample. PMID: 23512105
  13. SUMOylation of Pax7 is essential for neural crest development in chick embryos and for the inhibitory roles of Pax7 in C2C12-muscle differentiation. PMID: 23247248
  14. PAX7-FKHR fusion gene inhibits myogenic differentiation via NF-kappaB upregulation. PMID: 22374423
  15. Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury and disease. PMID: 21954137
  16. MyoD can play an active role in Alveolar rhabdomyosarcoma by augmenting Pax7-FKHR function. PMID: 21321994
  17. PAX7-FKHR gene fusion is prognostic indicator for alveolar rhabdomyosarcoma. PMID: 12039929
  18. expression of PAX7 is correlated with metastasis potential--REVIEW PMID: 12647804
  19. elevated PAX7 expression indicates myogenic satellite cell origin for embryonal rhabdomyosarcoma PMID: 12865925
  20. in the RD embryonal rhabdomyosarcoma cell line, expression of wild-type PAX7 is downregulated by PAX3-FKHR PMID: 15184910
  21. PAX 7-FKHR fusion transcripts were positive in 2/7 of alveolar rhabdomyosarcoma patients, and were negative in embryonal rhabdomyosarcoma and Control tumors. PMID: 17285543
  22. Results identified a differentiation-resistant skeletal muscle progenitor cell population that was Pax7+/desmin- and capable of self-renewal. PMID: 19097049
  23. TAT analysis of all trios revealed two SNPs in PAX7 showing significant excess maternal transmission Isolated cleft lip with or without cleft palate PMID: 19142206

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Involvement in disease
Rhabdomyosarcoma 2 (RMS2)
Subcellular Location
Nucleus.
Protein Families
Paired homeobox family
Database Links

HGNC: 8621

OMIM: 167410

KEGG: hsa:5081

STRING: 9606.ENSP00000364524

UniGene: Hs.113253

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