Recombinant Human Paternally-expressed gene 3 protein (PEG3), partial

Code CSB-YP867112HU
MSDS
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Source Yeast
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Code CSB-EP867112HU
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Source E.coli
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Code CSB-EP867112HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867112HU
MSDS
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Source Baculovirus
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Code CSB-MP867112HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEG3
Uniprot No.
Alternative Names
PEG3; KIAA0287; ZSCAN24; Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Induces apoptosis in cooperation with SIAH1A. Acts as a mediator between p53/TP53 and BAX in a neuronal death pathway that is activated by DNA damage. Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. Possesses a tumor suppressing activity in glioma cells.
Gene References into Functions
  1. Data suggest that PEG3 is required for TFEB induction and nuclear translocation in a VEGFR2- and AMPK-dependent manner for decorin/decorin receptor-evoked autophagy. (PEG3 = paternally expressed 3 protein; TFEB = transcription factor EB; VEGFR2 = vascular endothelial growth factor receptor-2; AMPK = AMP-activated protein kinase) PMID: 28798237
  2. Peg3 transcriptionally modulates BECN1 activity. PMID: 28174297
  3. Data indicate that a set of cis-regulatory motifs and corresponding trans factors that may be critical for the transcriptional regulation of the Peg3 (Paternally Expressed Gene 3) domain. PMID: 27104590
  4. In the current study, the authors have identified three alternative promoters for mouse Peg3 and one alternative promoter for human PEG3. PMID: 27075691
  5. A resident population of resident smooth muscle progenitor cells expressing PW1 was identified in pulmonary hypertension-associated vascular remodeling. PMID: 26838788
  6. PW1/Peg3 function is essential for conferring proper mesoangioblast competence and it's level in human mesoangioblasts may serve as a biomarker to identify donor populations for therapeutic application in muscular dystrophies. PMID: 25751651
  7. The PEG3-SCAN domain appears to constitute an assembly block, enabling PEG3 homo- or heterodimerization to control gene expression in a combinatorial fashion. PMID: 23936039
  8. Genetic translocations involving PEG3 gene is associated with mesenchymal hamartoma of the liver. PMID: 24120702
  9. we have unveiled a mechanism for a secreted proteoglycan in inducing Peg3, a master regulator of macroautophagy in endothelial cells PMID: 23798385
  10. Results show that a five percent increase in DNA methylation of PEG3 is associated with a 1.6-fold increase invasive cervical cancer (ICC) risk. PMID: 23418553
  11. None of the folate cycle genotypes in the mother or infant were related to the methylation of IGF2, PEG3, or LINE-1. PMID: 23151531
  12. PEG3 gene is imprinted, with preferential expression from the paternal allele in human placenta. PMID: 11331620
  13. mammalian imprinting utilizes Peg3/Pw1 to co-opt the Wnt pathway, thereby regulating development and glioma growth PMID: 20064927
  14. Peg3/Pw1 is a mediator between p53 and Bax in DNA damage-induced neuronal death PMID: 11943780
  15. These results support a model in which pEg3 contributes to the control of progression through mitosis by phosphorylation of the CDC25 phosphatases. PMID: 15908796
  16. Loss of PEG3 expression may be a frequent event in gynecologic cancers. Given the known role of PEG3 in p53-mediated apoptosis, it is possible that PEG3 functions as a tumor suppressor. PMID: 16023706
  17. These results suggest that pEg3 might play a role at the cell cortex during mitosis. PMID: 16159311
  18. Down-regulation of PEG3 due to loss of heterozygosity and promoter methylation is associated with ovarian cancers PMID: 18286529
  19. abnormal regulation of PEG3 is associated with several glioma subtypes and that it plays an important role in tumorigenesis. PMID: 19367087
  20. PEG3 imprinted domain of humans, cows, and mice contains differing numbers of differentially methylated regions (DMR), but the PEG3-CpG island is the only DMR that is conserved among these three species. PMID: 19397955

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Krueppel C2H2-type zinc-finger protein family
Tissue Specificity
Brain, glial cells, astrocytes, embryo, placenta, testis, ovary and uterus. In the placenta it is found in the layer of villous cytotrophoblast cells while in the ovary it is found in the cells of the ovarian stroma including the thecal layers around the
Database Links

HGNC: 8826

OMIM: 601483

KEGG: hsa:5178

STRING: 9606.ENSP00000326581

UniGene: Hs.731875

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