Recombinant Human Polynucleotide 5'-hydroxyl-kinase NOL9 (NOL9)

Code CSB-YP725557HU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP725557HU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP725557HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP725557HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP725557HU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
NOL9
Uniprot No.
Alternative Names
NOL9Polynucleotide 5'-hydroxyl-kinase NOL9; EC 2.7.1.-; Nucleolar protein 9
Species
Homo sapiens (Human)
Expression Region
2-702
Target Protein Sequence
ADSGLLLKR GSCRSTWLRV RKARPQLILS RRPRRRLGSL RWCGRRRLRW RLLQAQASGV DWREGARQVS RAAAARRPNT ATPSPIPSPT PASEPESEPE LESASSCHRP LLIPPVRPVG PGRALLLLPV EQGFTFSGIC RVTCLYGQVQ VFGFTISQGQ PAQDIFSVYT HSCLSIHALH YSQPEKSKKE LKREARNLLK SHLNLDDRRW SMQNFSPQCS IVLLEHLKTA TVNFITSYPG SSYIFVQESP TPQIKPEYLA LRSVGIRREK KRKGLQLTES TLSALEELVN VSCEEVDGCP VILVCGSQDV GKSTFNRYLI NHLLNSLPCV DYLECDLGQT EFTPPGCISL LNITEPVLGP PFTHLRTPQK MVYYGKPSCK NNYENYIDIV KYVFSAYKRE SPLIVNTMGW VSDQGLLLLI DLIRLLSPSH VVQFRSDHSK YMPDLTPQYV DDMDGLYTKS KTKMRNRRFR LAAFADALEF ADEEKESPVE FTGHKLIGVY TDFAFRITPR NRESHNKILR DLSILSYLSQ LQPPMPKPLS PLHSLTPYQV PFNAVALRIT HSDVAPTHIL YAVNASWVGL CKIQDDVRGY TNGPILLAQT PICDCLGFGI CRGIDMEKRL YHILTPVPPE ELRTVNCLLV GAIAIPHCVL KCQRGIEGTV PYVTTDYNFK LPGASEKIGA REPEEAHKEK PYRRPKFCRK MK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Related Products

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA.
Gene References into Functions
  1. we present the nol9sa1022/sa1022 mutant, a novel zebrafish ribosomopathy model, which recapitulates key human disease characteristics. PMID: 26624285
  2. The polynucleotide kinase activity of Nol9 is required for efficient generation of the 5.8S and 28S ribosomal RNAs from the 32S precursor. PMID: 21063389
Subcellular Location
Nucleus. Nucleus, nucleolus. Note=Colocalizes with pre-60S rRNP particles.
Protein Families
Clp1 family, NOL9/GRC3 subfamily
Database Links

HGNC: 26265

KEGG: hsa:79707

STRING: 9606.ENSP00000366934

UniGene: Hs.59425

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1