Recombinant Human Postacrosomal sheath WW domain-binding protein (WBP2NL)

Code CSB-YP750315HU
MSDS
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Source Yeast
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Code CSB-EP750315HU
MSDS
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Source E.coli
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Code CSB-EP750315HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750315HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP750315HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WBP2NL
Uniprot No.
Alternative Names
WBP2NL; PAWPPostacrosomal sheath WW domain-binding protein; WW domain-binding protein 2-like
Species
Homo sapiens (Human)
Expression Region
1-309
Target Protein Sequence
MAVNQSHTEN RRGALIPNGE SLLKRSPNVE LSFPQRSEGS NVFSGRKTGT LFLTSYRVIF ITSCSISDPM LSFMMPFDLM TNLTVEQPVF AANFIKGTIQ AAPYGGWEGQ ATFKLVFRNG DAIEFAQLMV KAASAAARGF PLRTLNDWFS SMGIYVITGE GNMCTPQMPC SVIVYGAPPA GYGAPPPGYG APPAGYGAQP VGNEGPPVGY RASPVRYGAP PLGYGAPPAG YGAPPLGYGA PPLGYGTPPL GYGAPPLGYG APPAGNEGPP AGYRASPAGS GARPQESTAA QAPENEASLP SASSSQVHS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in meotic resumption and pronuclear formation, mediated by a WW domain-signaling pathway during fertilization.
Gene References into Functions
  1. Taken into account that WBP2NL/PAWP gene and protein levels and distribution did not correlate with fertilization rates, this study questions the interest of WBP2NL/PAWP protein and gene expression analysis in sperm cells as a prognostic factor for the outcome of ICSI cycles PMID: 28444612
  2. High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCzeta, PAWP, and TR-KIT were low in globozoospermic individuals PMID: 27089467
  3. Conclude that human PAWP is neither sufficient nor necessary for the Ca(2+) oscillations that initiate mammalian oocyte activation at fertilization. PMID: 26116451
  4. WBP2NL might play roles as an anti-apoptotic factor or co-activator to promote breast cancer cell survival and proliferation. PMID: 25417742
  5. Demonstrate significant correlations between sperm PAWP levels and both fertilization rates and normal embryonic development after intracytoplasmic sperm injection. PMID: 24907910

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Tissue Specificity
Expressed in testis.
Database Links

HGNC: 28389

OMIM: 610981

KEGG: hsa:164684

STRING: 9606.ENSP00000328800

UniGene: Hs.655541

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