Recombinant Human Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (HOGA1)

Code CSB-YP771464HU
MSDS
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Source Yeast
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Code CSB-EP771464HU
MSDS
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Source E.coli
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Code CSB-EP771464HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP771464HU
MSDS
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Source Baculovirus
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Code CSB-MP771464HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HOGA1
Uniprot No.
Alternative Names
HOGA1; C10orf65; DHDPSL; 4-hydroxy-2-oxoglutarate aldolase; mitochondrial; EC 4.1.3.16; Dihydrodipicolinate synthase-like; DHDPS-like protein; Probable 2-keto-4-hydroxyglutarate aldolase; Probable KHG-aldolase; Protein 569272
Species
Homo sapiens (Human)
Expression Region
26-327
Target Protein Sequence
ASGEG KKVDIAGIYP PVTTPFTATA EVDYGKLEEN LHKLGTFPFR GFVVQGSNGE FPFLTSSERL EVVSRVRQAM PKNRLLLAGS GCESTQATVE MTVSMAQVGA DAAMVVTPCY YRGRMSSAAL IHHYTKVADL SPIPVVLYSV PANTGLDLPV DAVVTLSQHP NIVGMKDSGG DVTRIGLIVH KTRKQDFQVL AGSAGFLMAS YALGAVGGVC ALANVLGAQV CQLERLCCTG QWEDAQKLQH RLIEPNAAVT RRFGIPGLKK IMDWFGYYGG PCRAPLQELS PAEEEALRMD FTSNGWL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the final step in the metabolic pathway of hydroxyproline.
Gene References into Functions
  1. Results show that HOGA1 harboring mutations found in primary hyperoxaluria is thermally unstable and targeted for proteolytic degradation leading to an absolute loss of function. PMID: 27096395
  2. Among the seven patients identified with HOGA1 mutations the median onset of clinical symptoms was 1.8 years. Five patients initially presented with urolithiasis, and two other patients presented with urinary tract infection. PMID: 25972204
  3. Our results strongly suggest HOGA1 as a major cause of PH, indicate a greater genetic heterogeneity of hyperoxaluria, and point to a favorable outcome of type III in the context of PH despite incomplete or absent biochemical remission PMID: 22781098
  4. seven different mutations were identified in primary hyperoxaluria type 3 PMID: 22391140
  5. Two crystal forms of DHDPSL were obtained, both of which diffracted X-rays to approximately 2.0 A resolution PMID: 22232173
  6. hHOGA utilizes a type I aldolase reaction mechanism, but employs novel residue interactions for substrate binding PMID: 21998747
  7. Detection of HOGA1 variants in idiopathic calcium oxalate urolithiasis also suggests HOGA1 may be a predisposing factor for this condition PMID: 21896830
  8. DHDPSL is the gene encoding 4-hydroxy-2-oxoglutarate aldolase, catalyzing the final step in the metabolic pathway of hydroxyproline. PMID: 20797690

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Involvement in disease
Hyperoxaluria primary 3 (HP3)
Subcellular Location
Mitochondrion.
Protein Families
DapA family
Database Links

HGNC: 25155

OMIM: 613597

KEGG: hsa:112817

STRING: 9606.ENSP00000359680

UniGene: Hs.180346

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