Recombinant Human Probable methyltransferase C20orf7, mitochondrial (C20orf7)

Code CSB-YP713138HU
MSDS
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Source Yeast
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Code CSB-EP713138HU
MSDS
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Source E.coli
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Code CSB-EP713138HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP713138HU
MSDS
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Source Baculovirus
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Code CSB-MP713138HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NDUFAF5
Uniprot No.
Alternative Names
C20orf7; chromosome 20 open reading frame 7; CT007_HUMAN; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; NDUFAF5; Probable methyltransferase C20orf7; mitochondrial
Species
Homo sapiens (Human)
Expression Region
37-345
Target Protein Sequence
GSTS PRTLNIFDRD LKRKQKNWAA RQPEPTKFDY LKEEVGSRIA DRVYDIPRNF PLALDLGCGR GYIAQYLNKE TIGKFFQADI AENALKNSSE TEIPTVSVLA DEEFLPFKEN TFDLVVSSLS LHWVNDLPRA LEQIHYILKP DGVFIGAMFG GDTLYELRCS LQLAETEREG GFSPHISPFT AVNDLGHLLG RAGFNTLTVD TDEIQVNYPG MFELMEDLQG MGESNCAWNR KALLHRDTML AAAAVYREMY RNEDGSVPAT YQIYYMIGWK YHESQARPAE RGSATVSFGE LGKINNLMPP GKKSQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. Acts by mediating hydroxylation of 'Arg-111' of NDUFS7. May also have methyltransferase activity (Probable).
Gene References into Functions
  1. However, similar to another family member, RdmB, it catalyzes the introduction of a hydroxyl group, in the case of NDUFAF5, into Arg-73 in the NDUFS7 subunit of human complex I PMID: 27226634
  2. analysis of the combined OXPHOS complex I and IV defect, due to mutated complex I assembly factor C20ORF7 PMID: 21607760
  3. A new cause of Leigh syndrome can be a defect in early complex I assembly due to C20orf7 mutations. PMID: 19542079
  4. C20orf7 is crucial in the assembly of complex I and mutations in C20orf7 cause mitochondrial disease PMID: 18940309
Involvement in disease
Mitochondrial complex I deficiency (MT-C1D); Leigh syndrome (LS)
Subcellular Location
Mitochondrion inner membrane.
Protein Families
Methyltransferase superfamily
Database Links

HGNC: 15899

OMIM: 252010

KEGG: hsa:79133

STRING: 9606.ENSP00000367346

UniGene: Hs.472165

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