Recombinant Human Profilin-2 (PFN2)

Code CSB-YP017825HU
MSDS
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Source Yeast
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Code CSB-EP017825HU
MSDS
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Source E.coli
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Code CSB-EP017825HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017825HU
MSDS
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Source Baculovirus
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Code CSB-MP017825HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PFN2
Uniprot No.
Alternative Names
D3S1319E; PFL; PFN 2; Pfn2; PROF2_HUMAN; Profilin II; Profilin-2; Profilin2; ProfilinII
Species
Homo sapiens (Human)
Expression Region
1-140
Target Protein Sequence
MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLTLGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Gene References into Functions
  1. Study indicated that PFN2 may function as a negative regulator of colorectal cancer (CRC) metastasis and, thus, may represent a potential target for CRC therapy. PMID: 30015842
  2. High PFN2 expression might serve as a valuable predictor for poor overall survival of HNSC. DNA amplification and hypomethylation might be two mechanisms of PFN2 dysregulation. PMID: 29322815
  3. PFN2 gene, related to regulation of actin cytoskeleton in protein complex F, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis. PMID: 29642183
  4. PFN2 has a novel role in promoting Esophageal squamous cell carcinoma progression and metastasis. PMID: 27188458
  5. Study demonstrated that miR30a inhibits epithelial-mesenchymal transition (EMT) and invasion in highly invasive non-small cell lung cancer (NSCLC) cell lines via targeting PFN2 suggesting that miR30a with PFN2 may play an essential role in the development of NSCLC by modulating EMT and cell invasion. PMID: 28405690
  6. Disease causing mutations in inverted formin 2 regulate its binding to G-actin, F-actin capping protein (CapZ alpha-1) and profilin 2. PMID: 26764407
  7. Therefore, this study indicates that profilin 2 affects the metastatic potential and stemness of colorectal CSCs by regulating EMT- and stemness-related proteins. PMID: 25964982
  8. Pfn2 suppresses the recruitment of HDAC1 to Smad2 and Smad3 promoters, increasing their expression, and, in turn, enhancing TGFB1 induced epithelial-mesenchymal transformation and VEGF and CTGF production. PMID: 26354229
  9. Oral squamous cell carcinomas with weak PFN2 expression were associated with a significantly worse prognosis than strongly expressed tumours. PMID: 21725608
  10. These findings raise the possibility that Rgl3 mediates interaction between Ras/Rap-family proteins and profilin II, an important activator of actin polymerization. PMID: 17382517

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Profilin family
Tissue Specificity
Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
Database Links

HGNC: 8882

OMIM: 176590

KEGG: hsa:5217

STRING: 9606.ENSP00000239940

UniGene: Hs.91747

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