Recombinant Human Protein S100-A3 (S100A3)

Code CSB-YP020631HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP020631HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020631HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP020631HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
S100A3
Uniprot No.
Alternative Names
Protein S 100E; Protein S-100E; Protein S100 A3; Protein S100-A3; S100 calcium binding protein A3; S100 calcium-binding protein A3; S100a3; S100E; S10A3_HUMAN
Species
Homo sapiens (Human)
Expression Region
2-101
Target Protein Sequence
ARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation.
Gene References into Functions
  1. S100A3 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  2. One disulfide bridge is between Cys30 in the N-terminal pseudo-EF-hand and Cys68 in the C-terminal EF-hand (SS1), and another disulfide bridge attaches Cys99 in the C-terminal coil structure to Cys81 in helix IV (SS2). PMID: 21377473
  3. crystal structure of S100A3 at 1.7-A resolution PMID: 12045193
  4. structure was solved by MIRAS phasing PMID: 12136135
  5. Purification and characterization of the S100A3 protein from human hair cuticles. PMID: 12470658
  6. cytoplasmic S100A3 within the cuticular layer is mostly co-localized with the type III isoform of peptidylarginine deiminase (PAD3) PMID: 18083705

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Subcellular Location
Cytoplasm.
Protein Families
S-100 family
Tissue Specificity
Skin specific, specifically expressed at the inner endocuticle of hair fibers.
Database Links

HGNC: 10493

OMIM: 176992

KEGG: hsa:6274

STRING: 9606.ENSP00000357701

UniGene: Hs.557609

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