Recombinant Human DDB1- and CUL4-associated factor 1 (DCAF1), partial

1. Data suggest that HIV-1 vpr mediates polyubiquitination of HLTF by directly loading it onto C-terminal WD40 domain of DCAF1 in complex the CRL4, an E3 ubiquitin ligase. (vpr = vpr gene product of Human immunodeficiency virus 1; HLTF = human helicase like transcription factor; DCAF1 = human Vpr (HIV-1) binding protein; CRL4 = human E3 ubiquitin ligase CRL4) PMID: 29079575
2. The study suggests that DCAF1 is involved in hepatitis C virus (HCV) replication through regulation of miR-122, and thus provides new insights into the interaction between HCV and the host cell. PMID: 29327233
3. RNA interference-mediated knockdown of differentially regulated host factors identified Vpr binding protein (VprBP) as proviral host factor because its downregulation inhibited efficient propagation of seasonal influenza A virus. PMID: 28289176
4. High VPRBP expression is associated with high-grade serous ovarian cancer. PMID: 28416635
5. Together, these results characterize a novel postintegration restriction of HIV-1 replication in monocyte-derived dendritic cells and show that the interaction of Vpr with the DCAF1/DDB1 E3 ubiquitin ligase complex and the yet-to-be-identified host factor might alleviate this restriction by inducing transcription from the viral long terminal repeat. PMID: 28424288
6. This study presents the crystal structure of the DDB1-DCAF1-HIV-1-Vpr-uracil-DNA glycosylase (cyclin U) complex. PMID: 27571178
7. VprBP transcription was repressed by cellular miRNA-1236, which contributes to HIV-1 restriction in monocytes. miR-1236 inhibitors enhanced translation of VprBP and promoted infection. miR-1236 mimetics suppressed translation of VprBP. PMID: 24932481
8. MCM10 is the natural substrate of the Cul4-DDB1[VprBP] E3 ubiquitin ligase whose degradation is regulated by VprBP, but Vpr enhances the proteasomal degradation of MCM10 by interacting with VprBP. PMID: 26032416
9. Vpr downregulated APOBEC3G through Vpr-binding protein (VprBP)-mediated proteasomal degradation, and further confirmed that the reduction of APOBEC3G encapsidation associated with Vpr was due to Vpr's degradation-inducing activity. PMID: 25200749
10. Vpr and Vpx share a highly similar DCAF1-binding motif, they interact with a different set of residues in DCAF1. PMID: 25499532
11. VprBP, but not DDB1, directly binds to TET2-catalytic domain. PMID: 25557551
12. CD44 cytoplasmic tail cleaved by RIP could release DCAF1 from merlin by competing for binding to the merlin FERM domain, which results in the inhibition of merlin-mediated suppression of tumorigenesis. PMID: 24912773
13. The identification of Vpr mutants which associate with DCAF1 but only poorly with DDB1 suggests that DCAF1 is necessary but is not sufficient for the Vpr association with DDB1-containing E3 ligase complex. PMID: 24912982
14. Data indicate that DCAF1 protein folds into a beta-hairpin structure and binds to the F3 lobe of neurofibromin 2 (Merlin) FERM domain. PMID: 24706749
15. The predicted beta-propeller conformation of DCAF1 is likely to be critical for Vpr association. PMID: 24558487
16. Our findings establish VprBP as a major kinase responsible for H2AT120p in cancer cells and suggest that VprBP inhibition could be a new strategy for the development of anticancer therapeutics. PMID: 24140421
17. the crystal structure of a ternary complex of Vpx with the human E3 ligase substrate adaptor DCAF1 and the carboxy-terminal region of human SAMHD1 PMID: 24336198
18. As a molecular adaptor, Vpr enhanced the interaction between TERT and the VPRBP substrate receptor of the DYRK2-associated EDD-DDB1-VPRBP E3 ligase complex, resulting in increased ubiquitination of TERT. PMID: 23612978
19. Promoter-localized deacetylation of H3 tails is a prerequisite for VprBP to tether and act as a bona fide inhibitor at p53 target genes. PMID: 22184063
20. This review focuses on Vpr and its HIV2/SIV counterparts, Vpx and Vpr, which all engage the DDB1.Cullin4 ubiquitin ligase complex through the DCAF1 adaptor protein. PMID: 20347598
21. Study concludes that Merlin suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1). PMID: 20178741
22. demonstration that a novel human gene, KIAA0800, is preferentially expressed in the testis and is transactivated by Sox9 PMID: 12111997
23. Hence, this chimeric peptide (TEAM-VP) constitutes the first example of a virus-derived mitochondriotoxic compound as a candidate to kill selectively tumor neo-endothelia. PMID: 16888644
24. Recruitment of cytoplasmic protein Vpr binding protein (VprBP) by HIV-1 regulatory protein Vpr is essential for its cytostatic activity. PMID: 17314515
25. The Cul4-DDB1[VprBP] E3 ubiquitin ligase complex is identified as the downstream effector of lentiviral Vpr for the induction of cell cycle arrest in G2 phase. PMID: 17609381
26. Anti-tumor activity mediated by protein and peptide transduction of HIV viral protein R is reported. PMID: 19029839

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HGNC: 30911

KEGG: hsa:9730

STRING: 9606.ENSP00000393183

UniGene: Hs.716623