Recombinant Human Protein cereblon(CRBN)

Code CSB-BP842761HU
Size US$2644
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names CRBN
Uniprot No. Q96SW2
Research Area Neuroscience
Species Homo sapiens (Human)
Source Baculovirus
Expression Region 1-442aa
Target Protein Sequence MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 52.5 kDa
Protein Length Full Length
Tag Info C-terminal 9xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

Does it exist in its properly folded state such that it is biologically active? What buffer is being used?

A:
Thanks for your inquiry.1. We haven't tested the activity of this protein. This protein has been ordered for more than 10 times but we haven't received any feedback about the activity yet.
This literature mentions co-expression using the BP system and the expressed protein is used in subsequent studies.
(Recombinant baculovirus of ZZ-His-CRBN and DDB1-StrepTag (ST) were generated and amplified using Bac-to-Bac baculovirus expression system from Invitrogen in Sf9 insect cells.
ZZ-His-CRBN and DDB1-ST were co-expressed in High Five (Tni) insect in 10L wave bags at 27 ºC using un-supplemented ESF921 media from Expression Systems. )
Here is the link of the literature: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496085/
The protein we provide is full length and the expression host used by BP system is Sf9, meanwhile, this protein is purified under mild conditions, which should have activity in theory.
2. Our default storage buffer is Tris-based buffer, 50% glycerol. If you have special requirement for the storage buffer, please tell us and we will try to satisfy.
Q:

I have one more questions about CSB-BP842761HU protein.
What is the concentration of the remaining 90ug product?

A:
Thanks for your inquiry. The 90ug stock protein is liquid form, concentration 0.1mg/ml.
Buffer: 0.2 μm sterile filtered 20 mM Tris-HCl,0.5M NaCl,pH 8.0,50%glycerol
 Customer Reviews

Target Data

Function Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
Involvement in disease Mental retardation, autosomal recessive 2A (MRT2A)
Subcellular Location Cytoplasm, Nucleus, Membrane, Peripheral membrane protein
Protein Families CRBN family
Tissue Specificity Widely expressed. Highly expressed in brain.
Database Links

HGNC: 30185

OMIM: 607417

KEGG: hsa:51185

STRING: 9606.ENSP00000231948

UniGene: Hs.18925

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