Code | CSB-BP842761HU |
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Size | $472 |
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The gene fragment corresponding to the 1-442aa of the human CRBN protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with C-terminal 9xHis tag. The Baculovirus was transformed with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as C-terminal 9xHis-tagged fusion. This recombinant human CRBN protein's purity is greater than 85% assayed by SDS-PAGE. The CRBN protein ran to a band of about 56 kDa molecular weight on the gel, indicating a glycosylated form of the protein.
Cereblon (CRBN) is a common direct target of thalidomide and related compounds and works as a Cullin Ring 4 E3 ubiquitin ligase (CRL4) with DDB1, CUL4, and ROC1. CRBN the molecular target of lenalidomide and pomalidomide, is a substrate receptor of the cullin ring E 3 ubiquitin ligase complex, CRL 4 CRBN. In early reports, Researchers identifed that a novel cereblon modulator recruits GSPT1 to the CRL4-CRBN ubiquitin ligase. The substrate specificity of CRL4CRBN is modulated by thalidomide-related compounds. Ablation ofCRBNinduces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway. The CRBN, CUL4A and DDB1 expression predicts the response to immunomodulatory drugs and survival of multiple myeloma patients. Cereblon modulator CC-885 inducesCRBN-dependent ubiquitination and degradation of CDK4 in multiple myeloma.
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Does it exist in its properly folded state such that it is biologically active? What buffer is being used?
I have one more questions about CSB-BP842761HU protein.
What is the concentration of the remaining 90ug product?