Recombinant Human Proto-oncogene DBL(MCF2) ,partial

Code CSB-YP013579HU
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Source Yeast
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Code CSB-EP013579HU
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Source E.coli
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Code CSB-EP013579HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013579HU
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Source Baculovirus
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Code CSB-MP013579HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MCF2
Uniprot No. P10911
Alternative Names MCF2; DBLProto-oncogene DBL; Proto-oncogene MCF-2) [Cleaved into: MCF2-transforming protein; DBL-transforming protein]
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42.
Gene References into Functions
  1. Results support a role for Dbl oncogene in mammary epithelial cell differentiation and transformation and suggest the relevance of GEF deregulation in tumor onset and progression. PMID: 25723869
  2. Phosphoinositide 3-kinase C2beta regulates RhoA and the actin cytoskeleton through an interaction with Dbl PMID: 22984590
  3. Dbl is regulated by hamartin through association with ezrin. PMID: 21712385
  4. proto-oncogene dbl is not a major target for sporadic testicular germ cell tumors PMID: 19373475
  5. data describe 4 splicing variants of the human DBL (MCF-2) proto-oncogene that show different tissue distributions and guanine nucleotide exchange factor specificities PMID: 12445822
  6. provides fundamental data on the structure of Mcf-2, which regulates a variety of cellular signaling pathways PMID: 13679059
  7. These data suggest a mechanism whereby precise spatial guanine nucleotide exchange of Cdc42 by Dbl is dependent on functional ERM proteins and is important for directional cell migration. PMID: 17538024
  8. Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain PMID: 17606614
  9. Nm23-H1 can negatively regulate cell migration and tumor metastasis by modulating the activity of Cdc42 and possibly other Rho family members through interaction with Dbl-1 PMID: 18728402

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Involvement in disease MCF2 and DBL represent two activated versions of the same proto-oncogene.
Subcellular Location Cytoplasm, SUBCELLULAR LOCATION: Isoform 1: Membrane, SUBCELLULAR LOCATION: Isoform 3: Membrane
Protein Families MCF2 family
Tissue Specificity Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, int
Database Links

HGNC: 6940

OMIM: 311030

KEGG: hsa:4168

STRING: 9606.ENSP00000430276

UniGene: Hs.387262

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