Recombinant Human Serine/threonine-protein kinase Nek11 (NEK11)

Code CSB-YP844052HU
MSDS
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Source Yeast
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Code CSB-EP844052HU
MSDS
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Source E.coli
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Code CSB-EP844052HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844052HU
MSDS
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Source Baculovirus
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Code CSB-MP844052HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NEK11
Uniprot No.
Alternative Names
4932416N14Rik; FLJ23495; NEK11; NEK11_HUMAN; NEK11S/L; Never in mitosis A-related kinase 11; NIMA (never in mitosis gene a) related kinase 11; NIMA (never in mitosis gene a)- related kinase 11; NIMA related kinase 11L ; NIMA related kinase 11S; NIMA-related kinase 11; NimA-related protein kinase 11; Serine/threonine-protein kinase Nek11
Species
Homo sapiens (Human)
Expression Region
1-645
Target Protein Sequence
MLKFQEAAKC VSGSTAISTY PKTLIARRYV LQQKLGSGSF GTVYLVSDKK AKRGEELKVL KEISVGELNP NETVQANLEA QLLSKLDHPA IVKFHASFVE QDNFCIITEY CEGRDLDDKI QEYKQAGKIF PENQIIEWFI QLLLGVDYMH ERRILHRDLK SKNVFLKNNL LKIGDFGVSR LLMGSCDLAT TLTGTPHYMS PEALKHQGYD TKSDIWSLAC ILYEMCCMNH AFAGSNFLSI VLKIVEGDTP SLPERYPKEL NAIMESMLNK NPSLRPSAIE ILKIPYLDEQ LQNLMCRYSE MTLEDKNLDC QKEAAHIINA MQKRIHLQTL RALSEVQKMT PRERMRLRKL QAADEKARKL KKIVEEKYEE NSKRMQELRS RNFQQLSVDV LHEKTHLKGM EEKEEQPEGR LSCSPQDEDE ERWQGREEES DEPTLENLPE SQPIPSMDLH ELESIVEDAT SDLGYHEIPE DPLVAEEYYA DAFDSYCEES DEEEEEIALE RPEKEIRNEG SQPAYRTNQQ DSDIEALARC LENVLGCTSL DTKTITTMAE DMSPGPPIFN SVMARTKMKR MRESAMQKLG TEVFEEVYNY LKRARHQNAS EAEIRECLEK VVPQASDCFE VDQLLYFEEQ LLITMGKEPT LQNHL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Gene References into Functions
  1. contributes to response of colorectal cancer cells to genotoxic agents and is essential for survival either with or without exposure to DNA damage PMID: 26501353
  2. Results show that downregulation of NEK11 in drug resistant cells might contribute to drug resistance in ovarian cancer. PMID: 24969318
  3. NEK11 controls degradation of CDC25A by directly phosphorylating CDC25A on residues whose phosphorylation is required for beta-TrCP mediated CDC25A polyubiquitylation and degradation. PMID: 20090422
  4. Nek11 has a role in the S-phase checkpoint downstream of the caffeine-sensitive pathway PMID: 12154088
  5. Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells PMID: 15161910
  6. NEK11 regulates CDC25A degradation and the IR-induced G2/M checkpoint. PMID: 19734889

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Subcellular Location
Nucleus. Nucleus, nucleolus.
Protein Families
Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
Tissue Specificity
Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.
Database Links

HGNC: 18593

OMIM: 609779

KEGG: hsa:79858

STRING: 9606.ENSP00000372857

UniGene: Hs.657336

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