Recombinant Human Sorbin and SH3 domain-containing protein 2 (SORBS2), partial

Code CSB-YP022405HU
MSDS
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Source Yeast
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Code CSB-EP022405HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022405HU
MSDS
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Source Baculovirus
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Code CSB-MP022405HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SORBS2
Uniprot No.
Alternative Names
Arg binding protein 2; Arg-binding protein 2; Arg/Abl interacting protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; ArgBP2; KIAA0777; PRO0618; sorbin and SH3 domain containing 2; Sorbin and SH3 domain-containing protein 2; Sorbin; Sorbs2; SRBS2_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1. May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder.
Gene References into Functions
  1. By enhancing the stability of gene transcripts, SORBS2 suppresses ovarian cancer invasiveness and affects monocyte to myeloid-derived suppressor cell and M2-like macrophage polarization, eliciting a tumor-suppressive immune microenvironment. PMID: 29548303
  2. results thus contribute to the knowledge of the regulatory mechanism of HSF1 in down-regulating ArgBP2, providing new insight into the HSF1&MORC2-PRC2-ArgBP2 signaling pathway and a better understanding of their functions in gastric cancer cells. PMID: 29339121
  3. In spite of our finding that SORBS2 is clearly a component of the apical junction complex, it does not appear to be required for either normal tight- or adherens junction assembly, structure or function or for growth factor-mediated changes in tight junction dynamics PMID: 28961272
  4. SORBS2 transcription is activated by telomere position effect-over long distance upon telomere shortening in muscle cells from patients with facioscapulohumeral dystrophy PMID: 26359233
  5. ArgBP2 inhibits proliferation, migration, and invasion of gastric cancer cells.MORC2 down-regulates the ArgBP2 via histone methylation in gastric cancer cells. PMID: 26476214
  6. This study confirmed that SORBS2 as genetic modifiers of age at onset of Alzheimer disease. PMID: 26214276
  7. ArgBP2 interaction with alpha-actinin and actin stress fibers inhibits cell migration PMID: 25429109
  8. Tyrosine phosphorylation of ARGBP2 interferes with a SH3 mediated self-interaction, thereby controlling its panel of interacting partners and related functions. PMID: 24475245
  9. SORBS2 and TLR3 induce premature senescence in primary human fibroblasts and keratinocytes. PMID: 24165198
  10. Sarcoplasmic sorbin and SH3 domain-containing protein 2 is released from damaged cardiac tissue into the bloodstream upon lethal acute myocardial infarction. PMID: 24342996
  11. the expression and localization of hArgBP2 in osteosarcoma MG-63 cells PMID: 22394629
  12. In epithelial NMuMG cells, immunofluorescence analyses revealed localization of ArgBP2 at tight junctions and mutation analyses found a second SH3 domain to be important for ArgBP2 localization to the cell-cell contact sites. PMID: 22431180
  13. A co-localization of SORBS2 and eEF1A was evidenced at level of plasma membrane, thus suggesting the involvement of eEF1A1 in novel key signal transduction complexes. PMID: 21689717
  14. Reconstitution of SORBS2 expression resulted significant reduction in cell proliferation, colony formation and anchorage-independent growth in CaSki, HPKII and HaCaT cells, whereby anchorage-independent growth could only be investigated for CaSki cells PMID: 21602178
  15. Results indicate that ArgBP2 may play an important role in the assembly and maintenance of myofibrils in cardiomyocytes. PMID: 20886612
  16. CIP4 is a new ArgBP2 interacting protein that modulates the ArgBP2 mediated control of WAVE1 phosphorylation and cancer cell migration. PMID: 19631450
  17. complexation with Cbl mediates ubiquitination and degradation of c-Abl PMID: 12475393
  18. regulates cytoskeletal organization and signal transduction--review PMID: 12510380
  19. The sorbin N- and C-termini were localized in the human ArgBP2 gene locus; they are spliced to the 5' and 3' ends of the 95% homologous region; thus, a revised human sorbin nucleotide sequence is proposed. PMID: 15949647
  20. Loss of ArgBP2 is associated with pancreatic cancer. PMID: 18559503

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Subcellular Location
Cytoplasm, perinuclear region. Apical cell membrane. Cell junction, focal adhesion. Cell projection, lamellipodium.
Tissue Specificity
Abundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. In the panc
Database Links

HGNC: 24098

OMIM: 616349

KEGG: hsa:8470

STRING: 9606.ENSP00000284776

UniGene: Hs.481342

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