Recombinant Human Spermatid-specific manchette-related protein 1 (SMRP1)

Code CSB-YP847685HU
MSDS
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Source Yeast
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Code CSB-EP847685HU
MSDS
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Source E.coli
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Code CSB-EP847685HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847685HU
MSDS
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Source Baculovirus
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Code CSB-MP847685HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SMRP1
Uniprot No.
Alternative Names
SMRP1; C9orf24; CBE1Spermatid-specific manchette-related protein 1; Ciliated bronchial epithelial protein 1; Testis development protein NYD-SP22
Species
Homo sapiens (Human)
Expression Region
1-262
Target Protein Sequence
MFLFSRKTRT PISTYSDSYR APTSIKEVYK DPPLCAWEAN KFLTPGLTHT MERHVDPEAL QKMAKCAVQD YTYRGSISGH PYLPEKYWLS QEEADKCSPN YLGSDWYNTW RMEPYNSSCC NKYTTYLPRL PKEARMETAV RGMPLECPPR PERLNAYERE VMVNMLNSLS RNQQLPRITP RCGCVDPLPG RLPFHGYESA CSGRHYCLRG MDYYASGAPC TDRRLRPWCR EQPTMCTSLR APARNAVCCY NSPAVILPIS EP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in spermatogenesis. May be involved in differentiation or function of ciliated cells.
Gene References into Functions
  1. Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. PMID: 28601408
  2. CBE1 may play an important role in the differentiation and/or function of ciliated cells in human airways PMID: 15242845
  3. Chronological regulation of CBE1/Cbe1 expression during pulmonary differentiation suggests involvement in ciliogenesis, with an additional role during early lung development. PMID: 19213785
Subcellular Location
Cytoplasm, cytoskeleton. Nucleus. Cytoplasm.
Protein Families
SMRP1 family
Tissue Specificity
Expressed in epithelial cells of nasal polyps and in intact epithelium of bronchial biopsies from subjects with and without asthma. Strong expression in columnar cells with little or no expression in basal epithelial cells along the basement membrane. Str
Database Links

HGNC: 19919

KEGG: hsa:84688

STRING: 9606.ENSP00000297623

UniGene: Hs.50334

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