Recombinant Human Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial (SUCLG2), partial

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Code CSB-EP846636HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP846636HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) SUCLG2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP846636HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) SUCLG2.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
SUCLG2
Uniprot No.
Research Area
Metabolism
Alternative Names
EC 6.2.1.4 ; G BETA; GBETA; GTP specific succinyl-CoA synthetase beta subunit; GTP specific succinyl-CoA synthetase subunit beta; GTP-specific succinyl-CoA synthetase subunit beta; mitochondrial; SCS betaG ; SCS-betaG; SUCB2_HUMAN; Succinate CoA ligase GDP forming beta subunit; Succinate-Coenzyme A ligase; GDP-forming; beta subunit; Succinyl CoA ligase GDP forming beta chain mitochondrial ; Succinyl CoA synthetase betaG chain; Succinyl-CoA ligase [GDP-forming] subunit beta; Succinyl-CoA ligase [GDP-forming] subunit beta; mitochondrial; Succinyl-CoA synthetase beta-G chain; SUCLG2
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
49-423aa
Target Protein Sequence
MSDNGVRVQRFFVADTANEALEAAKRLNAKEIVLKAQILAGGRGKGVFNSGLKGGVHLTKDPNVVGQLAKQMIGYNLATKQTPKEGVKVNKVMVAEALDISRETYLAILMDRSCNGPVLVGSPQGGVDIEEVAASNPELIFKEQIDIFEGIKDSQAQRMAENLGFVGPLKSQAADQITKLYNLFLKIDATQVEVNPFGETPEGQVVCFDAKINFDDNAEFRQKDIFAMDDKSENEPIENEAAKYDLKYIGLDGNIACFVNGAGLAMATCDIIFLNGGKPANFLDLGGGVKEAQVYQAFKLLTADPKVEAILVNIFGGIVNCAIIANGITKACRELELKVPLVVRLEGTNVQEAQKILNNSGLPITSAIDLEDAAK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
56.3kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Gene References into Functions
  1. SUCLG2 has a sensitivity of 75% and a specificity of 80% to distinguish follicular carcinoma from follicular adenoma PMID: 28709933
  2. The absence of SUCLA2 and SUCLG2 in human glia is in compliance with the presence of alternative pathways occurring in these cells, namely the GABA shunt and ketone body metabolism PMID: 25370487
  3. SUCLG2 has been identified as both a determinator of CSF amyloid beta-protein (1-42) levels and as attenuator of cognitive decline in Alzheimer's disease. PMID: 25027320
  4. Loss of SUCLG2 is associated with mitochondrial DNA depletion and results in mitochondrial depletion syndrome. PMID: 21295139
Subcellular Location
Mitochondrion.
Protein Families
Succinate/malate CoA ligase beta subunit family, GTP-specific subunit beta subfamily
Tissue Specificity
Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary.
Database Links

HGNC: 11450

OMIM: 603922

KEGG: hsa:8801

STRING: 9606.ENSP00000419325

UniGene: Hs.644919

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