Recombinant Human Target of EGR1 protein 1 (TOE1)

Code CSB-YP856942HU
MSDS
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Source Yeast
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Code CSB-EP856942HU
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Source E.coli
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Code CSB-EP856942HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856942HU
MSDS
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Source Baculovirus
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Code CSB-MP856942HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TOE1
Uniprot No.
Alternative Names
Target of EGR1 protein 1; target of EGR1; member 1 ; Toe1; TOE1_HUMAN
Species
Homo sapiens (Human)
Expression Region
2-510
Target Protein Sequence
AADSDDGAV SAPAASDGGV SKSTTSGEEL VVQVPVVDVQ SNNFKEMWPS LLLAIKTANF VAVDTELSGL GDRKSLLNQC IEERYKAVCH AARTRSILSL GLACFKRQPD KGEHSYLAQV FNLTLLCMEE YVIEPKSVQF LIQHGFNFNQ QYAQGIPYHK GNDKGDESQS QSVRTLFLEL IRARRPLVLH NGLIDLVFLY QNFYAHLPES LGTFTADLCE MFPAGIYDTK YAAEFHARFV ASYLEYAFRK CERENGKQRA AGSPHLTLEF CNYPSSMRDH IDYRCCLPPA THRPHPTSIC DNFSAYGWCP LGPQCPQSHD IDLIIDTDEA AAEDKRRRRR RREKRKRALL NLPGTQTSGE AKDGPPKKQV CGDSIKPEET EQEVAADETR NLPHSKQGNK NDLEMGIKAA RPEIADRATS EVPGSQASPN PVPGDGLHRA GFDAFMTGYV MAYVEVSQGP QPCSSGPWLP ECHNKVYLSG KAVPLTVAKS QFSRSSKAHN QKMKLTWGSS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing.
Gene References into Functions
  1. Human cells with reduced levels of TOE1 accumulated 3'-end-extended pre-snRNA. PMID: 28092684
  2. Antiviral potency of TOE1 and its cell-penetrating capability have been identified to lie within a 35-amino-acid region containing the nuclear localization sequence PMID: 26056259
  3. TOE1 has a role in the growth inhibitory activity of Egr1 PMID: 12562764
Involvement in disease
Pontocerebellar hypoplasia 7 (PCH7)
Subcellular Location
Nucleus, nucleolus. Nucleus speckle. Note=Localizes to nuclear speckles.
Protein Families
CAF1 family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 15954

OMIM: 613931

KEGG: hsa:114034

STRING: 9606.ENSP00000361162

UniGene: Hs.525091

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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