Recombinant Human Transcriptional activator protein Pur-beta (PURB)

Code CSB-YP847212HU
MSDS
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Source Yeast
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Code CSB-EP847212HU
MSDS
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Source E.coli
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Code CSB-EP847212HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847212HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP847212HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PURB
Uniprot No.
Alternative Names
Pur beta; purB; PURB_HUMAN; PURBETA; Purine rich element binding protein B; Purine-rich element-binding protein B; transcriptional activator protein Pur beta; Transcriptional activator protein Pur-beta
Species
Homo sapiens (Human)
Expression Region
2-312
Target Protein Sequence
ADGDSGSER GGGGGPCGFQ PASRGGGEQE TQELASKRLD IQNKRFYLDV KQNAKGRFLK IAEVGAGGSK SRLTLSMAVA AEFRDSLGDF IEHYAQLGPS SPEQLAAGAE EGGGPRRALK SEFLVRENRK YYLDLKENQR GRFLRIRQTV NRGGGGFGAG PGPGGLQSGQ TIALPAQGLI EFRDALAKLI DDYGGEDDEL AGGPGGGAGG PGGGLYGELP EGTSITVDSK RFFFDVGCNK YGVFLRVSEV KPSYRNAITV PFKAWGKFGG AFCRYADEMK EIQERQRDKL YERRGGGSGG GEESEGEEVD ED
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs.
Gene References into Functions
  1. Results showed that Purbeta ssDNA-binding activity was significantly increased in myeloid cells from AML patients suggesting that the enhanced ssDNA-binding activity of Purbeta in transformed myeloid cells may serve as a unique and measurable phenotypic trait for improving prognostic risk stratification in AML. PMID: 28834593
  2. site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric... PMID: 27064749
  3. findings offer insights into mechanism, energetics, and sequence of Purbeta repressor binding to a biologically relevant, contractile phenotype-regulating cis-element while revealing the thermodynamic confines of Purbeta-mediated effects on DNA structure PMID: 19496623
Subcellular Location
Nucleus.
Protein Families
PUR DNA-binding protein family
Tissue Specificity
Expressed in myocardium of heart failure patients.
Database Links

HGNC: 9702

OMIM: 608887

KEGG: hsa:5814

STRING: 9606.ENSP00000379051

UniGene: Hs.349150

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