Recombinant Human Tyrosine-protein kinase FRK (FRK)

Code CSB-YP008988HU
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Source Yeast
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Code CSB-EP008988HU
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Source E.coli
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Code CSB-EP008988HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008988HU
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Source Baculovirus
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Code CSB-MP008988HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FRK
Uniprot No.
Alternative Names
Beta cell Src homology tyrosine kinase; BSK; BSK/IYK; C85044; Frk; Frk fyn related kinase; FRK_HUMAN; FYN related kinase; FYN-related kinase; GASK; Gastrointestinal associated kinase; Gastrointestinal associated tyrosine kinase; GTK; GTK; mouse; homolog of; Intestine tyrosine kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; PTK5 protein tyrosine kinase 5; RAK; Tyrosine protein kinase FRK; Tyrosine-protein kinase FRK
Species
Homo sapiens (Human)
Expression Region
1-505
Target Protein Sequence
MSNICQRLWE YLEPYLPCLS TEADKSTVIE NPGALCSPQS QRHGHYFVAL FDYQARTAED LSFRAGDKLQ VLDTLHEGWW FARHLEKRRD GSSQQLQGYI PSNYVAEDRS LQAEPWFFGA IGRSDAEKQL LYSENKTGSF LIRESESQKG EFSLSVLDGA VVKHYRIKRL DEGGFFLTRR RIFSTLNEFV SHYTKTSDGL CVKLGKPCLK IQVPAPFDLS YKTVDQWEID RNSIQLLKRL GSGQFGEVWE GLWNNTTPVA VKTLKPGSMD PNDFLREAQI MKNLRHPKLI QLYAVCTLED PIYIITELMR HGSLQEYLQN DTGSKIHLTQ QVDMAAQVAS GMAYLESRNY IHRDLAARNV LVGEHNIYKV ADFGLARVFK VDNEDIYESR HEIKLPVKWT APEAIRSNKF SIKSDVWSFG ILLYEIITYG KMPYSGMTGA QVIQMLAQNY RLPQPSNCPQ QFYNIMLECW NAEPKERPTF ETLRWKLEDY FETDSSYSDA NNFIR
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Gene References into Functions
  1. our results present the first evidence that site-specific promoter methylation contributes to the repression of FRK more so in basal B breast cancers. PMID: 28077797
  2. Loss of FRK protein may be implicated into the tumorigenesis and cell motility/invasiveness of human cervical cancer. PMID: 27665472
  3. antagomir-1290 significantly inhibited the proliferation, clonogenicity, invasion, and migration of CD133(+) cells by targeting fyn-related Src family tyrosine kinase. PMID: 25783528
  4. our results demonstrate a combined mechanism for the anti-proliferative effects of FRK by inhibiting cyclin D1 nucleus accumulation and pRb phosphorylation in glioma cells. PMID: 24792491
  5. FRK over-expression promoted beta-catenin translocation to the plasma membrane, where it formed complex with N-cadherin. PMID: 24969324
  6. exome sequencing identified recurrent somatic FRK mutations that induce constitutive kinase activity, STAT3 activation, and cell proliferation sensitive to Src inhibitors PMID: 24735922
  7. Data indicaate that the Rak/Frk SH2 and SH3 domains collaborate to increase the Rak-EGFR interaction. PMID: 23318459
  8. The expression levels of FRK were significantly correlated with invasiveness in both Hep3B and HepG2 tumor cell lines. PMID: 23267173
  9. these results clearly indicate that FRK may play a protective role against the progression of glioma by suppressing cell migration and invasion PMID: 22790444
  10. Genetic variation near FRK/COL10A1 and VEGFA are associated with advanced age-related macular degeneration. PMID: 21665990
  11. Study searched for mutations in the FRK gene, a recently identified tumor suppressor gene, for the first time in NSCLC. Somatic FRK mutations were found at a very low frequency in NSCLCs. PMID: 21074287
  12. Breast cancer cell line proliferation blocked by the Src-related Rak tyrosine kinase PMID: 12569567
  13. The tyrosine kinases Brk/PTK6/Sik, Srm, Frk/Rak/Gtk/Iyk/Bsk, and Src42A/Dsrc41 have a low degree of sequence homology to other known kinases. The exon structure of these kinases, called the Brk family, is highly conserved and distinct. PMID: 12725532
  14. Three common Japanese polymorphisms of the Fyn kinase gene studied in 127 healthy controls and 182 sporadic Alzheimer's disease failed to demonstrate any significant difference. PMID: 15082191
  15. Rak functions as a tumor suppressor by regulating PTEN protein stability and function in breast and bone neoplasms. PMID: 19345329

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Subcellular Location
Cytoplasm. Nucleus. Note=Predominantly found in the nucleus, with a small fraction found in the cell periphery.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
Tissue Specificity
Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
Database Links

HGNC: 3955

OMIM: 606573

KEGG: hsa:2444

STRING: 9606.ENSP00000357615

UniGene: Hs.89426

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