Recombinant Human Ubiquitin-conjugating enzyme E2 variant 3 (UEVLD)

Code CSB-YP816897HU
MSDS
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Source Yeast
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Code CSB-EP816897HU
MSDS
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Source E.coli
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Code CSB-EP816897HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP816897HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP816897HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UEVLD
Uniprot No.
Alternative Names
UEVLD; UEV3Ubiquitin-conjugating enzyme E2 variant 3; UEV-3; EV and lactate/malate dehydrogenase domain-containing protein
Species
Homo sapiens (Human)
Expression Region
1-471
Target Protein Sequence
MEFDCEGLRR LLGKYKFRDL TVEELRNVNV FFPHFKYSMD TYVFKDSSQK DLLNFTGTIP VMYQGNTYNI PIRFWILDSH PFAPPICFLK PTANMGILVG KHVDAQGRIY LPYLQNWSHP KSVIVGLIKE MIAKFQEELP MYSLSSSDEA RQVDLLAYIA KITEGVSDTN SKSWANHENK TVNKITVVGG GELGIACTLA ISAKGIADRL VLLDLSEGTK GATMDLEIFN LPNVEISKDL SASAHSKVVI FTVNSLGSSQ SYLDVVQSNV DMFRALVPAL GHYSQHSVLL VASQPVEIMT YVTWKLSTFP ANRVIGIGCN LDSQRLQYII TNVLKAQTSG KEVWVIGEQG EDKVLTWSGQ EEVVSHTSQV QLSNRAMELL RVKGQRSWSV GLSVADMVDS IVNNKKKVHS VSALAKGYYD INSEVFLSLP CILGTNGVSE VIKTTLKEDT VTEKLQSSAS SIHSLQQQLK L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Possible negative regulator of polyubiquitination.
Gene References into Functions
  1. identification, sequencing and analysis of cDNA in various colon carcinoma cell lines as well as normal and tumor samples from colon PMID: 12427560
Protein Families
Ubiquitin-conjugating enzyme family, UEV subfamily; LDH/MDH superfamily
Tissue Specificity
Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
Database Links

HGNC: 30866

OMIM: 610985

KEGG: hsa:55293

STRING: 9606.ENSP00000379500

UniGene: Hs.407991

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