Recombinant Human Unconventional myosin-IXa (MYO9A), partial

Code CSB-YP015354HU
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Source Yeast
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Code CSB-EP015354HU
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Source E.coli
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Code CSB-EP015354HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015354HU
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Source Baculovirus
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Code CSB-MP015354HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYO9A
Uniprot No.
Alternative Names
4732465J09Rik; C130068I12Rik; C230003M11; FLJ11061; FLJ13244; MGC71859; Myo9a; MYO9A variant protein; MYO9A_HUMAN; Myosin-IXa; MYR7; Unconventional myosin 9a; Unconventional myosin-9a
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Regulates Rho by stimulating it's GTPase activity in neurons. Required for the regulation of neurite branching and motor neuron axon guidance.
Gene References into Functions
  1. analysis of actin network self-organization by a monomeric myosin IXa PMID: 27956608
  2. MYO9A deficiency may affect the presynaptic motor axon, manifesting in congenital myasthenic syndrome. PMID: 27259756
  3. Vaccinia virus F11 promotes viral spread by binding to host myosin-9A and inhibit RhoA signaling. PMID: 23870313
  4. Depletion of myosin-IXA in collectively migrating cells led to altered organization of the actin cytoskeleton and tension-dependent disruption of cell-cell adhesions, followed by an inability to form new adhesions resulting in cell scattering. PMID: 22305756
Subcellular Location
Membrane; Single-pass membrane protein. Cytoplasm. Cell junction, synapse. Cell projection, growth cone.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Tissue Specificity
Found to be expressed in testis and placenta and at lower levels in all the examined tissues with the exception of liver. Isoform 5: Found in leukocytes but not in brain, retina or testis.
Database Links

HGNC: 7608

OMIM: 604875

KEGG: hsa:4649

STRING: 9606.ENSP00000348349

UniGene: Hs.513000

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