Recombinant Human Upstream-binding protein 1 (UBP1)

Code CSB-YP865191HU
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Source Yeast
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Code CSB-EP865191HU
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Source E.coli
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Code CSB-EP865191HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865191HU
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Source Baculovirus
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Code CSB-MP865191HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UBP1
Uniprot No.
Alternative Names
UBP1; LBP1; Upstream-binding protein 1; Transcription factor LBP-1
Species
Homo sapiens (Human)
Expression Region
1-540
Target Protein Sequence
MAWVLKMDEV IESGLVHDFD ASLSGIGQEL GAGAYSMSDV LALPIFKQED SSLPLDGETE HPPFQYVMCA ATSPAVKLHD ETLTYLNQGQ SYEIRMLDNR KMGDMPEING KLVKSIIRVV FHDRRLQYTE HQQLEGWKWN RPGDRLLDLD IPMSVGIIDT RTNPSQLNAV EFLWDPAKRT SAFIQVHCIS TEFTPRKHGG EKGVPFRIQV DTFKQNENGE YTDHLHSASC QIKVFKPKGA DRKQKTDREK MEKRTAHEKE KYQPSYDTTI LTEMRLEPII EDAVEHEQKK SSKRTLPADY GDSLAKRGSC SPWPDAPTAY VNNSPSPAPT FTSPQQSTCS VPDSNSSSPN HQGDGASQTS GEQIQPSATI QETQQWLLKN RFSSYTRLFS NFSGADLLKL TKEDLVQICG AADGIRLYNS LKSRSVRPRL TIYVCREQPS STVLQGQQQA ASSASENGSG APYVYHAIYL EEMIASEVAR KLALVFNIPL HQINQVYRQG PTGIHILVSD QMVQNFQDES CFLFSTVKAE SSDGIHIILK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2. Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
Gene References into Functions
  1. Data revealed that dimers composed of LBP-1a and LBP-1c were re-formed probably by a partner-exchange of LBP-1b-containing heterodimers. PMID: 19751393
  2. LBP-1b is an important SF1-independent transcriptional activator stimulating P450scc expression in human placental JEG-3 cells, whereas LBP-9 modulates the action of LBP-1b, exerting both positive and negative effects PMID: 15471945
  3. LBP-1b and LBP-9 both stimulate LBP-32/MGR promoter activity. PMID: 18004979
  4. UBP1 is a critical blood pressure determinant using a combination of mouse and human genetics. PMID: 19662162
Subcellular Location
Nucleus.
Protein Families
Grh/CP2 family, CP2 subfamily
Tissue Specificity
Expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines.
Database Links

HGNC: 12507

OMIM: 609784

KEGG: hsa:7342

STRING: 9606.ENSP00000283628

UniGene: Hs.729120

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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