Recombinant Human Zinc finger protein 322 (ZNF322)

Code CSB-YP757767HU
MSDS
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Source Yeast
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Code CSB-EP757767HU
MSDS
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Source E.coli
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Code CSB-EP757767HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP757767HU
MSDS
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Source Baculovirus
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Code CSB-MP757767HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ZNF322
Uniprot No.
Alternative Names
FLJ23393; HCG12; HLA complex group 12; OTTHUMP00000215823; OTTHUMP00000215824; Z322A_HUMAN; Zinc finger protein 322; Zinc finger protein 322A; Zinc finger protein 388; Zinc finger protein 489; ZNF322; ZNF322A; ZNF388; ZNF489
Species
Homo sapiens (Human)
Expression Region
1-402
Target Protein Sequence
MYTSEEKCNQ RTQKRKIYNV CPRKGKKIFI HMHEIIQIDG HIYQCLECKQ NFCENLALIM CERTHTGEKP YKCDMCEKTF VQSSDLTSHQ RIHNYEKPYK CSKCEKSFWH HLALSGHQRT HAGKKFYTCD ICGKNFGQSS DLLVHQRSHT GEKPYLCSEC DKCFSRSTNL IRHRRTHTGE KPFKCLECEK AFSGKSDLIS HQRTHTGERP YKCNKCEKSY RHRSAFIVHK RVHTGEKPYK CGACEKCFGQ KSDLIVHQRV HTGEKPYKCL ECMRSFTRSA NLIRHQATHT HTFKCLEYEK SFNCSSDLIV HQRIHMEEKP HQWSACESGF LLGMDFVAQQ KMRTQTEELH YKYTVCDKSF HQSSALLQHQ TVHIGEKPFV CNVSEKGLEL SPPHASEASQ MS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.
Gene References into Functions
  1. Silencing ZNF322 recapitulated the cellular and molecular effects seen upon miR-4317 overexpression. PMID: 28880489
  2. ZNF322A overexpression transcriptionally dysregulates genes involved in cell growth and motility therefore contributes to lung tumorigenesis and poor prognosis PMID: 26279304
  3. These results suggest that ZNF322 is a member of the zinc-finger transcription factor family and may act as a positive regulator in gene transcription mediated by the MAPK signaling pathways. PMID: 15555580
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Krueppel C2H2-type zinc-finger protein family
Tissue Specificity
Ubiquitous. Highly expressed in heart and skeletal muscle.
Database Links

HGNC: 23640

OMIM: 610847

KEGG: hsa:79692

STRING: 9606.ENSP00000418897

UniGene: Hs.126280

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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