Recombinant Human adenovirus C serotype 2 E1B protein, large T-antigen (E1B)

Code CSB-YP365887HIK
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Source Yeast
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Code CSB-EP365887HIK
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Source E.coli
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Code CSB-EP365887HIK-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP365887HIK
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Source Baculovirus
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Code CSB-MP365887HIK
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
E1B
Uniprot No.
Alternative Names
E1BE1B 55 kDa protein; E1B-55K; E1B protein; large T-antigen; E1B-495R
Species
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Expression Region
1-495
Target Protein Sequence
MERRNPSERG VPAGFSGHAS VESGGETQES PATVVFRPPG NNTDGGATAG GSQAAAAAGA EPMEPESRPG PSGMNVVQVA ELFPELRRIL TINEDGQGLK GVKRERGASE ATEEARNLTF SLMTRHRPEC VTFQQIKDNC ANELDLLAQK YSIEQLTTYW LQPGDDFEEA IRVYAKVALR PDCKYKISKL VNIRNCCYIS GNGAEVEIDT EDRVAFRCSM INMWPGVLGM DGVVIMNVRF TGPNFSGTVF LANTNLILHG VSFYGFNNTC VEAWTDVRVR GCAFYCCWKG VVCRPKSRAS IKKCLFERCT LGILSEGNSR VRHNVASDCG CFMLVKSVAV IKHNMVCGNC EDRASQMLTC SDGNCHLLKT IHVASHSRKA WPVFEHNILT RCSLHLGNRR GVFLPYQCNL SHTKILLEPE SMSKVNLNGV FDMTMKIWKV LRYDETRTRC RPCECGGKHI RNQPVMLDVT EELRPDHLVL ACTRAEFGSS DEDTD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest. E1B-55K also inactivates TP53 transcription-factor activity by binding its transactivation domain. E1B-55K also functions as a SUMO1 E3 ligase for TP53 which causes the latter to be sequestered in promyelocytic leukemia (PML) nuclear bodies thereby contributing to maximal inhibition of TP53 function.
Subcellular Location
Host nucleus. Host cytoplasm.
Protein Families
Adenoviridae E1B 55 kDa protein family
Database Links

KEGG: vg:2652981

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