Product Details

Purity

>85% (SDS-PAGE)

Target Names

UL49

Uniprot No.

P10233

Alternative Names

UL49; Tegument protein VP22

Species

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)

Expression Region

1-301

Target Protein Sequence

MTSRRSVKSG PREVPRDEYE DLYYTPSSGM ASPDSPPDTS RRGALQTRSR QRGEVRFVQY DESDYALYGG SSSEDDEHPE VPRTRRPVSG AVLSGPGPAR APPPPAGSGG AGRTPTTAPR APRTQRVATK APAAPAAETT RGRKSAQPES AALPDAPAST APTRSKTPAQ GLARKLHFST APPNPDAPWT PRVAGFNKRV FCAAVGRLAA MHARMAAVQL WDMSRPRTDE DLNELLGITT IRVTVCEGKN LLQRANELVN PDVVQDVDAA TATRGRSAAS RPTERPRAPA RSASRPRRPV E

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Tegument protein that plays different roles during the time course of infection. Participates in both the accumulation of viral mRNAs and viral protein translation at late time of infection. Modulates the RNase activity of the virion host shutoff protein UL41 probably to ensure necessary levels of key cellular mRNAs and proteins. Plays a role in microtubule reorganization that occurs after viral infection by stabilizing microtubule network. Finally, may prevent nucleosomal deposition onto the viral genome by interacting with and inhibiting host SET.

Gene References into Functions

herpes simplex virus 1 tegument protein VP22 inhibits AIM2-dependent inflammasome activation. VP22 interacts with AIM2 and prevents its oligomerization, an initial step in AIM2 inflammasome activation. PMID: 29447697
findings indicate that HSV-1 VP22 acts as an antagonist of IFN signaling to persistently evade host innate antiviral responses. PMID: 29793952
These findings suggest that VP22 is required (but not sufficient) for the inhibition of CD1d-mediated antigen presentation in herpes simplex virus type 1 infection. PMID: 27327902
The authors have determined the crystal structure of the conserved core domain of VP22, which reveals an elongated dimer with several potential protein-protein interaction regions and a peptide-binding site. PMID: 26068188
HSV-1 VP22 is a significant neurovirulence factor in vivo. PMID: 22357273
while RNF10 and WDR42A or VP22 alone showed distinct subcellular localization patterns, RNF10 and WDR42A were relocated when co-expressed with VP22 or its homologues; these potential host cell factors of VP22 might expand the list of host targets of VP22 PMID: 21424732
We have identified an interaction between VP22, an abundant tegument protein and the cytoplasmic tail of glycoprotein E (gE). PMID: 16997344
nexpression of the UL41 vhs protein in the absence of U(L)49 protein is lethal. PMID: 17670820
VP22 can easily transport between different cells but nuclear accumulation of the protein is not common in all of the recipient cells. PMID: 18051705
The authors conclude that nuclear VP22 targets unique subnuclear structures early (<6hpi) during herpes simplex virus 1 (HSV-1) infection. PMID: 18584907
VP22 was required for optimal synthesis of the late viral proteins gE and gD and the immediate-early protein ICP0 but did not have discernible effects on accumulation of the immediate-early proteins ICP4 or ICP27. PMID: 18987147
role of the tegument protein VP22 in viral replication PMID: 18989638
Small mutations within the gE binding domain of VP22 inhibit the VP22-gE interaction in infected cells. PMID: 19279114
These results indicate that the last fifteen amino acids of VP22 participate in virion targeting during HSV-1 infection. PMID: 19307008

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Subcellular Location

Virion tegument. Host cytoplasm. Host nucleus. Host Golgi apparatus. Note=One of the most abundant tegument protein (about 2000 copies per virion). Localizes in the cytoplasm at 8 hours postinfection and in the nucleus at 16 hours postinfection. During virion morphogenesis, this protein probably accumulates at the trans-Golgi where secondary envelopment occurs.

Protein Families

Alphaherpesvirinae VP22 tegument protein family

Database Links

KEGG: vg:2703417

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Recombinant Human herpesvirus 1 Tegument protein VP22 (UL49)

Product Details

Customer Reviews and Q&A

Target Background

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