Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Rev(rev)

Product Type Recombinant Protein
Uniprot No. P18803
Species Human immunodeficiency virus type 1 group M subtype D (isolate NDK) (HIV-1)
Gene Names rev
Protein Names Recommended name: Protein Rev Alternative name(s): ART/TRS Anti-repression transactivator Regulator of expression of viral proteins
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm.
Subcellular Location Host nucleus, host nucleolus, Host cytoplasm
Protein Families HIV-1 REV protein family

Product Details for Different Expression Systems

Product Name Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Rev(rev),Yeast
Code CSB-YP323053HLJ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGRSGDSDE NLLKAIRLIK FLYQSNPPPS PEGTRQARRN RRRRWRARQR QIHSIGERII CTFLGRPEEP VPLQLPPLER LNLNCSEDCG TSGTQGVGSP QIPVEPPAVL ESGTEE
Expression Region 1-116
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Rev(rev),E.coli
Code CSB-EP323053HLJ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGRSGDSDE NLLKAIRLIK FLYQSNPPPS PEGTRQARRN RRRRWRARQR QIHSIGERII CTFLGRPEEP VPLQLPPLER LNLNCSEDCG TSGTQGVGSP QIPVEPPAVL ESGTEE
Expression Region 1-116
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Rev(rev),Baculovirus
Code CSB-BP323053HLJ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGRSGDSDE NLLKAIRLIK FLYQSNPPPS PEGTRQARRN RRRRWRARQR QIHSIGERII CTFLGRPEEP VPLQLPPLER LNLNCSEDCG TSGTQGVGSP QIPVEPPAVL ESGTEE
Expression Region 1-116
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Rev(rev),Mammalian cell
Code CSB-MP323053HLJ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MAGRSGDSDE NLLKAIRLIK FLYQSNPPPS PEGTRQARRN RRRRWRARQR QIHSIGERII CTFLGRPEEP VPLQLPPLER LNLNCSEDCG TSGTQGVGSP QIPVEPPAVL ESGTEE
Expression Region 1-116
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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