Recombinant Human parvovirus B19 Minor capsid protein VP1, partial

Very nice to receive your inquiry. As to your question, pls check our reply as follows:
CSB-MP362102HPM Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 20% glycerol
As you can see, actually the glycerol concentration of these two items are already below our default shipping buffer 50% glycerol.
According to your feedback, since you already received these two items, does you want to change the buffer by yourself or need to order again ?
If you want to order again, we recommend you to choose the lyophilized form, the buffer before lyophilization will not contain any glycerol.
If you want to change the buffer or remove the glycerol by ourself, you can choose to remove the glycerol by dialysis or change the PBS buffer.
In addition, probably you can inform your application or requirements in advance next time, so that we can provide and supply the products according to his demand.
Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 10% glycerol

Very nice to receive your inquiry. CSB-MP362102HPM
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 91470
Abs 0.1% (=1 g/l) 1.504, assuming all pairs of Cys residues form cystines

Very nice to receive your inquiry. 1.Sorry, I'm afraid we can't provide any useful information, since we haven't validated the stability in PBS buffer, But if your customer has already changed the buffer to PBS (got rid of glycerol) and stored them at -20C, will your customer use them after a long period ?
in theory, if the protein is already stable in it, this operation doesn't have any problem, and they will also be stable when stored at -20C.

2.Regarding resolubilizing the proteins, the reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins.
(Dissolve completely. The concentration could depend on customers' experiment demand.) It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted.
Generally, we recommend 0.1-1.0mg/ml concentration. If customer has special requirements for concentration, then they could dilute the protein according to their experiment demand.
If the stability is not good after reconstitution, could it be possible to make aliquots?
Yes, we can make aliquots according to your request, pls remark your requirement when placing the order.
3.Yes, we can provide these two proteins in lyophilized format, generally, we will do reconstitution test for all lyophilized proteins before the shipment, if we find they are not soluble following lyophilization, then we will provide them in liquid form. We can provide the protein with your desired buffer (PBS buffer), pls remark your requirement when placing the order.