Product Details

Purity

>85% (SDS-PAGE)

Target Names

Uniprot No.

Q9Q0L7

Alternative Names

NS; Nuclear export protein; NEP; Non-structural protein 2; NS2

Species

Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd)

Expression Region

1-121

Target Protein Sequence

MDSNTITSFQ DILQRMSKMQ LESSSVDLNG MITQFERLKI YRDSLGESMM RMGDLHSLQN RNATWRNELS QKFEEIRWLI AECRNILTKT ENSFEQITFL QALQLLLEVE SEIRTFSFQL I

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs bound to the protein M1. Since protein M1 is not available in large quantities before late stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the host nucleus until sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enter the host cytoplasm only when associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.

Gene References into Functions

Ubiquitin and SUMO compete for the same lysine (K242) on influenza A virus M1 and the interaction of viral NS2 with human AIMP2 facilitates the switch of the M1 modification from ubiquitination to SUMOylation, thus increasing viral replication. PMID: 25320310
Although NEP stimulates viral RNA synthesis by binding to the viral polymerase, its function during nuclear export implicates interaction with viral ribonucleoprotein (vRNP)-associated M1. PMID: 24891509
Adaptive mutations in NEP compensate for defective H5N1 RNA replication in mammalian cells. PMID: 22549831
The high pathogenic strains of the avian influenza H5N1 virus isolated in Kazakhstan have NS of different genotypes. A/swan/Mangystau/3/2006 strain is of NS2A genotype that is typical for Gs/Gd-like strains. PMID: 21338659
Deletion of nucleotides of NS gene from 263 to 277 sites increases H5N1 pathogenesis in chicken. PMID: 16736596
results indicate that the NS1 gene is critical for the pathogenicity of avian influenza virus in chickens and that the amino acid residue Ala149 correlates with the ability of these viruses to antagonize interferon induction in chick embryo fibroblasts PMID: 16971424
Amino acid from 80 to 84 involved in virus resistance against cytokinewas was deleted in NS1. PMID: 17672298
15-nucleotide deletion of NS gene from site 263 to 277 increased the virulence of H5N1 viruses in chickens and mice. PMID: 18317917
NS1 protein of H5N1 down-regulates the expression level of IP-10. PMID: 19031697
During the process of serial passages in ducks, only the NS gene developed non-synonymous substitutions, which caused two mutations in NS1 protein (Val23Ala and Leu207Pro) and one in NS2 (Phe55Leu). PMID: 19802744

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Subcellular Location

Virion. Host nucleus.

Protein Families

Influenza viruses NEP family

Database Links

KEGG: vg:3654622

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Recombinant Influenza A virus Nuclear export protein (NS)

Product Details

Customer Reviews and Q&A

Target Background

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