Recombinant Laccaria bicolor Flap endonuclease 1-A(FEN11)

Code CSB-YP534586LLZ
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Source Yeast
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Code CSB-EP534586LLZ
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Source E.coli
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Code CSB-EP534586LLZ-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP534586LLZ
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Source Baculovirus
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Code CSB-MP534586LLZ
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names FEN11
Uniprot No. B0DSN9
Alternative Names FEN11; LACBIDRAFT_254074Flap endonuclease 1-A; FEN-1-A; EC 3.1.-.-; Flap structure-specific endonuclease 1-A
Species Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)
Expression Region 1-394
Target Protein Sequence MGIKGLTGLL SQHAPKAIQE HEIKTLFGRK VAIDASMSIY QFLIAVRQKD GELLTNDAGE TTSHLMGLFY RTLRIVENGI KPAYIFDGKP PELKKGVLSK RLERREEAKE EGEEAKETGT VEDVDRFSRR TVKVTREHNE ECRRLLRLMG IPVVIAPSEA EAQCAELARG GKVYAAGSED MDTLTFNAPI LFRHLTFSEA KKQPISEINL KEALEGLDMN MSQFIDLCIL LGCDYLEPIK GVGPKSALKL IREYGGLKGV VKHLRENSGC RGMQIPDEWP WEEAKKIFEK PDVLPADEVE LEWTNPDVDG LVQFLVKEKG FNEDRVRKGA EKLQKFLNSK QQGRLDGFFS VKPKEKAAAP APVGKAKGKG KVDAKAKGTK RKVGFLNSMS GPLA
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Subcellular Location Nucleus, nucleolus, Nucleus, nucleoplasm, Mitochondrion
Protein Families XPG/RAD2 endonuclease family, FEN1 subfamily
Database Links

KEGG: lbc:LACBIDRAFT_254074

STRING: 486041.XP_001887033.1

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