Recombinant Laccaria bicolor Flap endonuclease 1-B(FEN12)

Product Type Recombinant Protein
Uniprot No. B0E412
Species Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)
Gene Names FEN12
Protein Names Recommended name: Flap endonuclease 1-B Short name= FEN-1-B EC= 3.1.-.- Alternative name(s): Flap structure-specific endonuclease 1-B
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Subcellular Location Nucleus, nucleolus, Nucleus, nucleoplasm, Mitochondrion
Protein Families XPG/RAD2 endonuclease family, FEN1 subfamily
Database Links

KEGG: lbc:LACBIDRAFT_192371

STRING: 486041.XP_001890929.1

Product Details for Different Expression Systems

Product Name Recombinant Laccaria bicolor Flap endonuclease 1-B(FEN12),Yeast
Code CSB-YP534587LLZ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGIKGLTGLL SQHAPKAIKE IKTLFGRKVA IDASMSIYQF LIAVRQKDGE LLTNDAGETT RYLMGLFYRT LRIVENGIKP AYIFDGKPPE LKKGVLSKRL ERREEAKEEG EEAKETGTVE DVDRFSRRTV KVTREHNQEC QRLLRLMGIP VVIAPSEAEA QCAELARGGK VYIALYYAAG SEDMDTLTFN APILFRHLTF SEAKKQPISE INLKEALEGP LYSFPTFIDL CILLGCDYLE PIKGVGPKSA LKLIREYGGL KGVVKHLREK AATQKAAQAA VESDEESEHE EEDEPAPTSD VEMPDPVEED QDGEEEAEPK SSQATPKKKK ASSKTKEKRK GKGGGGMQIP EEWPWEEAKK IFKRPSHVHR DLEWTNPDVD GLVQFLVNEK GFNEDRVRKG AEKLQKFLNS KQQGRLDGFF SVKPKEKAAA PAPVGKAKGK GKIDAKAKGT KRKVDEKAES SAGKKPRKK
Expression Region 1-469
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Laccaria bicolor Flap endonuclease 1-B(FEN12),E.coli
Code CSB-EP534587LLZ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGIKGLTGLL SQHAPKAIKE IKTLFGRKVA IDASMSIYQF LIAVRQKDGE LLTNDAGETT RYLMGLFYRT LRIVENGIKP AYIFDGKPPE LKKGVLSKRL ERREEAKEEG EEAKETGTVE DVDRFSRRTV KVTREHNQEC QRLLRLMGIP VVIAPSEAEA QCAELARGGK VYIALYYAAG SEDMDTLTFN APILFRHLTF SEAKKQPISE INLKEALEGP LYSFPTFIDL CILLGCDYLE PIKGVGPKSA LKLIREYGGL KGVVKHLREK AATQKAAQAA VESDEESEHE EEDEPAPTSD VEMPDPVEED QDGEEEAEPK SSQATPKKKK ASSKTKEKRK GKGGGGMQIP EEWPWEEAKK IFKRPSHVHR DLEWTNPDVD GLVQFLVNEK GFNEDRVRKG AEKLQKFLNS KQQGRLDGFF SVKPKEKAAA PAPVGKAKGK GKIDAKAKGT KRKVDEKAES SAGKKPRKK
Expression Region 1-469
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Laccaria bicolor Flap endonuclease 1-B(FEN12),Baculovirus
Code CSB-BP534587LLZ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGIKGLTGLL SQHAPKAIKE IKTLFGRKVA IDASMSIYQF LIAVRQKDGE LLTNDAGETT RYLMGLFYRT LRIVENGIKP AYIFDGKPPE LKKGVLSKRL ERREEAKEEG EEAKETGTVE DVDRFSRRTV KVTREHNQEC QRLLRLMGIP VVIAPSEAEA QCAELARGGK VYIALYYAAG SEDMDTLTFN APILFRHLTF SEAKKQPISE INLKEALEGP LYSFPTFIDL CILLGCDYLE PIKGVGPKSA LKLIREYGGL KGVVKHLREK AATQKAAQAA VESDEESEHE EEDEPAPTSD VEMPDPVEED QDGEEEAEPK SSQATPKKKK ASSKTKEKRK GKGGGGMQIP EEWPWEEAKK IFKRPSHVHR DLEWTNPDVD GLVQFLVNEK GFNEDRVRKG AEKLQKFLNS KQQGRLDGFF SVKPKEKAAA PAPVGKAKGK GKIDAKAKGT KRKVDEKAES SAGKKPRKK
Expression Region 1-469
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Laccaria bicolor Flap endonuclease 1-B(FEN12),Mammalian cell
Code CSB-MP534587LLZ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGIKGLTGLL SQHAPKAIKE IKTLFGRKVA IDASMSIYQF LIAVRQKDGE LLTNDAGETT RYLMGLFYRT LRIVENGIKP AYIFDGKPPE LKKGVLSKRL ERREEAKEEG EEAKETGTVE DVDRFSRRTV KVTREHNQEC QRLLRLMGIP VVIAPSEAEA QCAELARGGK VYIALYYAAG SEDMDTLTFN APILFRHLTF SEAKKQPISE INLKEALEGP LYSFPTFIDL CILLGCDYLE PIKGVGPKSA LKLIREYGGL KGVVKHLREK AATQKAAQAA VESDEESEHE EEDEPAPTSD VEMPDPVEED QDGEEEAEPK SSQATPKKKK ASSKTKEKRK GKGGGGMQIP EEWPWEEAKK IFKRPSHVHR DLEWTNPDVD GLVQFLVNEK GFNEDRVRKG AEKLQKFLNS KQQGRLDGFF SVKPKEKAAA PAPVGKAKGK GKIDAKAKGT KRKVDEKAES SAGKKPRKK
Expression Region 1-469
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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