Recombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA1(ecfA1)

Code CSB-YP382860LND
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Source Yeast
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Code CSB-EP382860LND
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Source E.coli
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Code CSB-EP382860LND-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP382860LND
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Source Baculovirus
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Code CSB-MP382860LND
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ecfA1
Uniprot No. A2RI01
Alternative Names ecfA1; cbiO1; ecfA; llmg_0287Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA; EC 7.-.-.-
Species Lactococcus lactis subsp. cremoris (strain MG1363)
Expression Region 1-277
Target Protein Sequence MNKILEVENL VFKYEKESDV NQLNGVSFSI TKGEWVSIIG QNGSGKSTTA RLIDGLFEEF EGIVKIDGER LTAENVWNLR RKIGMVFQNP DNQFVGATVE DDVAFGMENQ GIPREEMIKR VDEALLAVNM LDFKTREPAR LSGGQKQRVA VAGIIALRPE IIILDESTSM LDPTGRSEIM RVIHEIKDKY HLTVLSITHD LDEAASSDRI LVMRAGEIIK EAAPSELFAT SEDMVEIGLD VPFSSNLMKD LRTNGFDLPE KYLSEDELVE LLADKLG
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. In this organism these probably include biotin, thiamine precursor, niacin, pantothenic acid, queuosine precursor, riboflavin and thiamine. Uptake of niacin or riboflavin into proteosomes containing EcfA1A2T and Niax or RibU has been demonstrated. Uptake requires hydrolyzable Mg-ATP and is substrate-specific; NiaX-containing proteosomes did not transport riboflavin.
Subcellular Location Cell membrane; Peripheral membrane protein.
Protein Families ABC transporter superfamily, Energy-coupling factor EcfA family
Database Links

KEGG: llm:llmg_0287

STRING: 416870.llmg_0287

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