Recombinant Lake Victoria marburgvirus Polymerase cofactor VP35 (VP35)

Code CSB-YP336033LBQ
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Source Yeast
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Code CSB-EP336033LBQ
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Source E.coli
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Code CSB-EP336033LBQ-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336033LBQ
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Source Baculovirus
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Code CSB-MP336033LBQ
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VP35
Uniprot No.
Alternative Names
VP35Polymerase cofactor VP35; Marburg VP35; mVP35
Species
Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Expression Region
1-329
Target Protein Sequence
MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC LMSKATSTDD IIWDQLIVKR TLADLLIPIN RQISDIQSTL SEVTTRVHEI ERQLHEITPV LKMGRTLEAI SKGMSEMLAK YDHLVISTGR TTAPAAAFDA YLNEHGVPPP QPAIFKDLGV AQQACSKGTM VKNATTDAAD KMSKVLELSE ETFSKPNLSA KDLALLLFTH LPGNNTPFHI LAQVLSKIAY KSGKSGAFLD AFHQILSEGE NAQAALTRLS RTFDAFLGVV PPVIRVKNFQ TVPRPSQKSL RAVPPNPTID KGWVCVYSSE QGETRALKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling.
Gene References into Functions
  1. In cell culture, Marburg virus infection results in a greater upregulation of IFN responses as compared to Ebolavirus infection, which correlates with differences in the efficiencies by which EBOV and MARV VP35s antagonize RIG-I signaling. PMID: 26876165
  2. Three important residues Arg271, Arg294, and Lys298 which makes the largest contribution for binding in VP35 lose their binding affinity significantly in mutants. PMID: 24459115
  3. Specific mutations in the IFN inhibitory domain (IID) of MARV VP35 that abrogate host immune responses. PMID: 25531184
  4. studies suggest VP35 can both coat the backbone and cap the ends of dsRNA, and that for MARV, coating of the dsRNA backbone may be an essential mechanism by which dsRNA is masked from backbone-sensing immune surveillance molecules PMID: 23028316
  5. The crystal structure of MARV VP35 IID in complex with 18-bp dsRNA reveals that despite the similar protein fold as EBOV VP35 IID, MARV VP35 IID interacts with the dsRNA backbone and not with blunt ends. PMID: 23185024

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Subcellular Location
Virion. Host cytoplasm.
Protein Families
Filoviridae polymerase cofactor VP35 family
Database Links

KEGG: vg:920948

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