Recombinant Leptospira biflexa serovar Patoc Ketol-acid reductoisomerase(ilvC)

Product Type Recombinant Protein
Uniprot No. B0SCQ5
Species Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames)
Gene Names ilvC
Protein Names Recommended name: Ketol-acid reductoisomerase EC= 1.1.1.86 Alternative name(s): Acetohydroxy-acid isomeroreductase Alpha-keto-beta-hydroxylacil reductoisomerase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
Protein Families Ketol-acid reductoisomerase family
Database Links

KEGG: lbf:LBF_0685

Product Details for Different Expression Systems

Product Name Recombinant Leptospira biflexa serovar Patoc Ketol-acid reductoisomerase(ilvC),Yeast
Code CSB-YP534872LOQ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MANIYYDDSC DLNLLKGKTI AVIGYGSQGH AQAQNMKDSG LKVIIGLRDG SKSVKEAKEA GFEVYNVAEA SKKADIIQIL APDTIQADMY KADIEPNLSE GKALVFSHGF NIHYDLITPP KNVDVYMVAP KGPGHLVRRV YTEGGGVPCL IAIYQDATGQ AKARALAHAS GVGGGRAGIL ETSFREETET DLFGEQAVLC GGVANLIMSG FETLTEAGYD PEIAYFECLH EVKLITDLIY EGGLARMRFS ISDTAEYGDY ISGPRVIDAG VKARMKDVLT DIQKDKGAAF AKRWMADTKA GYPEYKKLKE KNAAHPIEAV GTKLRSMMKW LAK
Expression Region 1-333
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Leptospira biflexa serovar Patoc Ketol-acid reductoisomerase(ilvC),E.coli
Code CSB-EP534872LOQ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MANIYYDDSC DLNLLKGKTI AVIGYGSQGH AQAQNMKDSG LKVIIGLRDG SKSVKEAKEA GFEVYNVAEA SKKADIIQIL APDTIQADMY KADIEPNLSE GKALVFSHGF NIHYDLITPP KNVDVYMVAP KGPGHLVRRV YTEGGGVPCL IAIYQDATGQ AKARALAHAS GVGGGRAGIL ETSFREETET DLFGEQAVLC GGVANLIMSG FETLTEAGYD PEIAYFECLH EVKLITDLIY EGGLARMRFS ISDTAEYGDY ISGPRVIDAG VKARMKDVLT DIQKDKGAAF AKRWMADTKA GYPEYKKLKE KNAAHPIEAV GTKLRSMMKW LAK
Expression Region 1-333
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Leptospira biflexa serovar Patoc Ketol-acid reductoisomerase(ilvC),Baculovirus
Code CSB-BP534872LOQ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MANIYYDDSC DLNLLKGKTI AVIGYGSQGH AQAQNMKDSG LKVIIGLRDG SKSVKEAKEA GFEVYNVAEA SKKADIIQIL APDTIQADMY KADIEPNLSE GKALVFSHGF NIHYDLITPP KNVDVYMVAP KGPGHLVRRV YTEGGGVPCL IAIYQDATGQ AKARALAHAS GVGGGRAGIL ETSFREETET DLFGEQAVLC GGVANLIMSG FETLTEAGYD PEIAYFECLH EVKLITDLIY EGGLARMRFS ISDTAEYGDY ISGPRVIDAG VKARMKDVLT DIQKDKGAAF AKRWMADTKA GYPEYKKLKE KNAAHPIEAV GTKLRSMMKW LAK
Expression Region 1-333
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Leptospira biflexa serovar Patoc Ketol-acid reductoisomerase(ilvC),Mammalian cell
Code CSB-MP534872LOQ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MANIYYDDSC DLNLLKGKTI AVIGYGSQGH AQAQNMKDSG LKVIIGLRDG SKSVKEAKEA GFEVYNVAEA SKKADIIQIL APDTIQADMY KADIEPNLSE GKALVFSHGF NIHYDLITPP KNVDVYMVAP KGPGHLVRRV YTEGGGVPCL IAIYQDATGQ AKARALAHAS GVGGGRAGIL ETSFREETET DLFGEQAVLC GGVANLIMSG FETLTEAGYD PEIAYFECLH EVKLITDLIY EGGLARMRFS ISDTAEYGDY ISGPRVIDAG VKARMKDVLT DIQKDKGAAF AKRWMADTKA GYPEYKKLKE KNAAHPIEAV GTKLRSMMKW LAK
Expression Region 1-333
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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