Recombinant Leptospira biflexa serovar Patoc tRNA modification GTPase MnmE(mnmE)

Product Type Recombinant Protein
Uniprot No. B0SJ24
Species Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)
Gene Names mnmE
Protein Names Recommended name: tRNA modification GTPase MnmE EC= 3.6.-.-
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.
Subcellular Location Cytoplasm
Protein Families TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, TrmE GTPase family
Database Links

KEGG: lbi:LEPBI_I0290

STRING: 456481.LEPBI_I0290

Product Details for Different Expression Systems

Product Name Recombinant Leptospira biflexa serovar Patoc tRNA modification GTPase MnmE(mnmE),Yeast
Code CSB-YP534881LOR
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIDTIAALST AQGPGAIGIL RVSGSLVMPI ALAVLEKNQK PLTETFLQNQ KRSAIFCDFV ENGKPLDQIV FFYFPAPNSY TGEDLAEFHL HGNPLLLKRA LHVLFEKGAR PAQKGEFTKR AYMNGKINLS GAEAISRLIE ARSKYELELA QKNVFGEITK LSSKIRSDLI SLKAECEAEI DFSTEDLTFE SLEERKNRMV ALKNLCSKLI KDSERAESYI LQSTVVLYGE PNTGKSSLMN LLIGKDRSII SDVPGTTRDY IAEELSLDGI PIRLVDTAGI RDTTDNIEQM GIERSKREAD SANVKLFLID TSLPFEKQSF LLKHKDRLFG SLIVANKIDS KHPSWHTESI HDIQEEFQLT ISEISCKTKQ GIPELLELLK SKLTSKDDTE DLVLLEDRQR YHIQKIESCL SEAIQLMENN APAEIYIQEI NVALHEIGQV NGVVENEEIL GRIFSKFCVG K
Expression Region 1-461
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Leptospira biflexa serovar Patoc tRNA modification GTPase MnmE(mnmE),E.coli
Code CSB-EP534881LOR
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIDTIAALST AQGPGAIGIL RVSGSLVMPI ALAVLEKNQK PLTETFLQNQ KRSAIFCDFV ENGKPLDQIV FFYFPAPNSY TGEDLAEFHL HGNPLLLKRA LHVLFEKGAR PAQKGEFTKR AYMNGKINLS GAEAISRLIE ARSKYELELA QKNVFGEITK LSSKIRSDLI SLKAECEAEI DFSTEDLTFE SLEERKNRMV ALKNLCSKLI KDSERAESYI LQSTVVLYGE PNTGKSSLMN LLIGKDRSII SDVPGTTRDY IAEELSLDGI PIRLVDTAGI RDTTDNIEQM GIERSKREAD SANVKLFLID TSLPFEKQSF LLKHKDRLFG SLIVANKIDS KHPSWHTESI HDIQEEFQLT ISEISCKTKQ GIPELLELLK SKLTSKDDTE DLVLLEDRQR YHIQKIESCL SEAIQLMENN APAEIYIQEI NVALHEIGQV NGVVENEEIL GRIFSKFCVG K
Expression Region 1-461
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Leptospira biflexa serovar Patoc tRNA modification GTPase MnmE(mnmE),Baculovirus
Code CSB-BP534881LOR
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIDTIAALST AQGPGAIGIL RVSGSLVMPI ALAVLEKNQK PLTETFLQNQ KRSAIFCDFV ENGKPLDQIV FFYFPAPNSY TGEDLAEFHL HGNPLLLKRA LHVLFEKGAR PAQKGEFTKR AYMNGKINLS GAEAISRLIE ARSKYELELA QKNVFGEITK LSSKIRSDLI SLKAECEAEI DFSTEDLTFE SLEERKNRMV ALKNLCSKLI KDSERAESYI LQSTVVLYGE PNTGKSSLMN LLIGKDRSII SDVPGTTRDY IAEELSLDGI PIRLVDTAGI RDTTDNIEQM GIERSKREAD SANVKLFLID TSLPFEKQSF LLKHKDRLFG SLIVANKIDS KHPSWHTESI HDIQEEFQLT ISEISCKTKQ GIPELLELLK SKLTSKDDTE DLVLLEDRQR YHIQKIESCL SEAIQLMENN APAEIYIQEI NVALHEIGQV NGVVENEEIL GRIFSKFCVG K
Expression Region 1-461
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Leptospira biflexa serovar Patoc tRNA modification GTPase MnmE(mnmE),Mammalian cell
Code CSB-MP534881LOR
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIDTIAALST AQGPGAIGIL RVSGSLVMPI ALAVLEKNQK PLTETFLQNQ KRSAIFCDFV ENGKPLDQIV FFYFPAPNSY TGEDLAEFHL HGNPLLLKRA LHVLFEKGAR PAQKGEFTKR AYMNGKINLS GAEAISRLIE ARSKYELELA QKNVFGEITK LSSKIRSDLI SLKAECEAEI DFSTEDLTFE SLEERKNRMV ALKNLCSKLI KDSERAESYI LQSTVVLYGE PNTGKSSLMN LLIGKDRSII SDVPGTTRDY IAEELSLDGI PIRLVDTAGI RDTTDNIEQM GIERSKREAD SANVKLFLID TSLPFEKQSF LLKHKDRLFG SLIVANKIDS KHPSWHTESI HDIQEEFQLT ISEISCKTKQ GIPELLELLK SKLTSKDDTE DLVLLEDRQR YHIQKIESCL SEAIQLMENN APAEIYIQEI NVALHEIGQV NGVVENEEIL GRIFSKFCVG K
Expression Region 1-461
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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