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Recombinant Listeria monocytogenes serovar 1/2a Actin assembly-inducing protein (actA), partial

Recombinant Listeria monocytogenes serovar 1/2a Actin assembly-inducing protein (actA), partial

^Unavailable

Code	CSB-YP330247LPY1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP330247LPY1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP330247LPY1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP330247LPY1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP330247LPY1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

actA

Uniprot No.

P33379

Alternative Names

actA; prtB; lmo0204; Actin assembly-inducing protein

Species

Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

The concentration of the E coli derived actA Recombinant Protein (cat#: CSB-EP330247LPY1; Lot#: 03653 is 0.3mg/mL. I would like to know if you have any advice or experience on how to concentrate the Acta protein? Or is it possible to lyophilize it? I think the concentration of 0.3mg/mL is too small. So if I want to try to concentrate it, can you please provide your feedback and recommendations without negatively affecting the protein.

Thanks for your feedback.
1. You could use Ultrafiltration Tube or Dialysis Bag to concentrate the protein.
2. You could perform dialysis to remove Glycerol, then the protein will be in buffer containing NO Glycerol (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), and finally lyophilize the protein.

Target Background

Function

Virulence factor required for host cell microfilament interaction. It induces actin assembly around the bacteria to allow it to move within the cytoplasm. It is involved in the actin polymerization process. It seems to act as a nucleator that induces the reorganization of the actin cytoskeleton.

Gene References into Functions

ActA plays a major role in listerial virulence, and its absence renders bacteria intracellularly immotile and essentially non-infectious.[review] PMID: 27726006
ActA associates to peptidoglycan, as revealed by cell wall proteomics of intracellular Listeria monocytogenes PMID: 21846725
ActA-mediated cell-to-cell spreading plays a major role in the vertical transmission of L. monocytogenes to the fetus in the murine model. PMID: 17118980

Subcellular Location

Cell membrane; Single-pass membrane protein.

Database Links

KEGG: lmo:lmo0204

STRING: 169963.lmo0204

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