Recombinant Methanococcus maripaludis Signal recognition 54 kDa protein(srp54)

Code CSB-YP389245MNP
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Source Yeast
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Code CSB-EP389245MNP
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Source E.coli
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Code CSB-EP389245MNP-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP389245MNP
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Source Baculovirus
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Code CSB-MP389245MNP
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names srp54
Uniprot No. A4FVX4
Alternative Names srp54; MmarC5_0025; Signal recognition particle 54 kDa protein; SRP54
Species Methanococcus maripaludis (strain C5 / ATCC BAA-1333)
Expression Region 1-450
Target Protein Sequence MLDKLGQNLS DALNKIKNAT FVDKKLVKEV IKDIQKALIQ SDVNVKLVFN MSKEIERKAI EEAPPKGLSK KEHIVKIVYD ELVKLLGETT QKLELDPSKK SVILLIGIQG SGKTTSAAKL ARYIQKKGLR PGLIAADVYR PAAYQQLKQL SEKINVPLFG DETRTKTPVD ITKEGMEKLK KVDVIIIDTA GRHKEEESLL AEMKEMKDLT NPNEIILVID GTLGQQAKNQ AKAFKESVSE IGSILVTKLD GSAKGGGALS AVAEINAPIK FIGTGEGVDN LEQFDPKKFI SRILGLGDLD SLLEKTEDIM DESTEESIDS ILKGKFTLIE LYAQLETISK MGPMKQILSM IPGMGGNLPK EAAQLTEDKL KRYKIMMDSM TLEEKENPEL IKTSRLQRIA KGAGVKQDEI KDLLKYYSTT KNAFGNLKRG KMPKMGGQMG QIMRQLMYKD
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.
Subcellular Location Cytoplasm
Protein Families GTP-binding SRP family, SRP54 subfamily
Database Links

KEGG: mmq:MmarC5_0025

STRING: 402880.MmarC5_0025

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