Recombinant Methanococcus vannielii tRNA-splicing endonuclease(endA)

Code CSB-YP409361MNS
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Source Yeast
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Code CSB-EP409361MNS
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Source E.coli
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Code CSB-EP409361MNS-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP409361MNS
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Source Baculovirus
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Code CSB-MP409361MNS
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names endA
Uniprot No. A6UPD9
Alternative Names endA; Mevan_0454tRNA-splicing endonuclease; EC 4.6.1.16; tRNA-intron endonuclease
Species Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)
Expression Region 1-177
Target Protein Sequence MMAKPKKTIP AKLSDERIVI YDKDGISRLN EKRYGELHEN FLSLSYVEAL YLVAKDWVLI RDKKNKILSF EELHEIAHVI DKKLCIRYLV YKDLRNRGYT VRTGLKYGSD FRLYERSNID EVHSKYLVKV FSEEIPCEIS EITGFVRVAH SVRKELVIAI VDADGSVVYY NMGYLKL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Protein Families TRNA-intron endonuclease family, Archaeal short subfamily
Database Links

KEGG: mvn:Mevan_0454

STRING: 406327.Mevan_0454

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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