Recombinant Methanosarcina mazei Flap endonuclease 1 (fen)

Code CSB-YP844389MSN
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Source Yeast
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Code CSB-EP844389MSN
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Source E.coli
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Code CSB-EP844389MSN-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844389MSN
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Source Baculovirus
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Code CSB-MP844389MSN
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
fen
Uniprot No.
Alternative Names
fen; MM_0906; Flap endonuclease 1; FEN-1; EC 3.1.-.-; Flap structure-specific endonuclease 1
Species
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Expression Region
1-338
Target Protein Sequence
MGTDIGDLLQ KRKIELSDLS NRVVAVDAFN TLHQFLSIIR QRDGSPLVNS QGKVTSHLSG LLYRTASLVE AGIKPVFVFD GKPPEMKTGT LNRRKEIRES SKEKWENAKA EGNLEAAYKY AQASSKVDQD IIEDSKYLLD IMGIPWIQAP CEGEAQAAHM VLKKDADCVA SQDYDSFLFG APTVVRNLAA TGKRKLPGKN VYVDVELEMI ELEETLDSLG INRDQLIDIA ICVGTDYNKG LEKVGPKTAL KLIKKHGNIH AVIREKGMEI EALDSIKELF THPDVTDDYE IKWGKPDSEK LINFLCDEND FSEDRVVKAA DRLKAASGAR QQTLDQWF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
Protein Families
XPG/RAD2 endonuclease family, FEN1 subfamily
Database Links

KEGG: mma:MM_0906

STRING: 192952.MM_0906

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