Recombinant Methanosarcina mazei Proteasome-activating nucleotidase (pan)

Code CSB-YP844388MSN
MSDS
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Source Yeast
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Code CSB-EP844388MSN
MSDS
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Source E.coli
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Code CSB-EP844388MSN-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844388MSN
MSDS
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Source Baculovirus
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Code CSB-MP844388MSN
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pan
Uniprot No.
Alternative Names
pan; MM_1006; Proteasome-activating nucleotidase; PAN; Proteasomal ATPase; Proteasome regulatory ATPase; Proteasome regulatory particle
Species
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Expression Region
1-420
Target Protein Sequence
MRSHLVKPGS VYDGIEPGEL GETTESVQDR VRQLESRNSF LEEQCSQIES EKRYLENQKI KYEREIRKLQ SELDRMKTSP LIIGTVIDVI KNDRIIVRSS NGPQFLVNVS QYIDEKKLLP GAKVALNQHT LAIAEVIPST EEPFVAAMEV IESVEVDYDQ IGGLDEQIQE LQEAVELPLT SPERFARIGI EPPKGVLLYG LPGTGKTLLA KAVAHRTNAT FIRVVGSELV QKYIGDGSKL VREIFEMARK KAPSIIFIDE LDSIAARRLN ETTGADREVQ RTLMQLLAEM DGFDKRKNIR IIAATNRPDV LDPAILRPGR FDRLVHVPMP GIEARGKILK IHCEKMTLAE DIDFKKLAKA TEGMSGADLK AIATEAGMFA VRRDKELVEM EEFLEAVEKV SMAADTQKMM PSNLPETMFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.
Subcellular Location
Cytoplasm.
Protein Families
AAA ATPase family
Database Links

KEGG: mma:MM_1006

STRING: 192952.MM_1006

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