Recombinant Methylibium petroleiphilum Queuine tRNA-ribosyltransferase (tgt)

Code CSB-YP019146MNX
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Source Yeast
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Code CSB-EP019146MNX
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Source E.coli
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Code CSB-EP019146MNX-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019146MNX
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Source Baculovirus
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Code CSB-MP019146MNX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tgt
Uniprot No.
Alternative Names
tgt; Mpe_A3733; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
Species
Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1)
Expression Region
1-375
Target Protein Sequence
MLRFDLLHTE GHARRGRLTL NHGVVETPIF MPVGTYGTVK GVMPASLEAM GAQIILGNTF HLWLRPGLDV LRQFGGLHRF ENWQRPILTD SGGFQVWSLG AMRKISEEGV KFASPVNGDK LFLTPETSMQ IQTVLNSDIV MQFDECTPYE TSGHLTTEQE ARASMELSLR WAARCKTEFA RLENPNALFG IVQGGMFEPL RQASLDALVA MDFPGYAIGG VSVGEPKDEM LRIMAHTPHR LPAHKPRYLM GVGTPEDLVE GVTQGVDLFD CVMPTRNARN GHLFTRHGDL RLRNARYKTD ERPIDESCTC TACNGFSRAY LHHLDRCGEM LGPMLTSIHN LHYFLNLMRE VREALDAGRF EAFRAQFRAD RARGV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Protein Families
Queuine tRNA-ribosyltransferase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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