Recombinant Methylocella silvestris Ketol-acid reductoisomerase(ilvC)

Product Type Recombinant Protein
Uniprot No. B8EKP4
Species Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Gene Names ilvC
Protein Names Recommended name: Ketol-acid reductoisomerase EC= 1.1.1.86 Alternative name(s): Acetohydroxy-acid isomeroreductase Alpha-keto-beta-hydroxylacil reductoisomerase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
Protein Families Ketol-acid reductoisomerase family
Database Links

KEGG: msl:Msil_3011

STRING: 395965.Msil_3011

Product Details for Different Expression Systems

Product Name Recombinant Methylocella silvestris Ketol-acid reductoisomerase(ilvC),Yeast
Code CSB-YP491148MTF
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDAD INLIKSKKVA IIGYGSQGHA HALNLRDSGV MDVSVALREG SATAAKAAGE GLKVLSVADA AKWADVVMML TPDELQGDIY SEHLAPNLKQ GAALLFAHGL NIHFNLIEPR KDLDVAMVAP KGPGHTVRSE YKRGGGVPCL IAIAQDASGN AHDIALSYAS AIGGGRAGII ETTFREECET DLFGEQVVLC GGLVELIRGG FETLVEAGYA PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VTGPRIVTPE TKAEMKRVLT DIQSGKFTRD WMLENKVNQT SFKATRAKAA SHQIEEVGAK LRAMMPWIGA NKLVDKAKN
Expression Region 1-339
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Methylocella silvestris Ketol-acid reductoisomerase(ilvC),E.coli
Code CSB-EP491148MTF
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDAD INLIKSKKVA IIGYGSQGHA HALNLRDSGV MDVSVALREG SATAAKAAGE GLKVLSVADA AKWADVVMML TPDELQGDIY SEHLAPNLKQ GAALLFAHGL NIHFNLIEPR KDLDVAMVAP KGPGHTVRSE YKRGGGVPCL IAIAQDASGN AHDIALSYAS AIGGGRAGII ETTFREECET DLFGEQVVLC GGLVELIRGG FETLVEAGYA PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VTGPRIVTPE TKAEMKRVLT DIQSGKFTRD WMLENKVNQT SFKATRAKAA SHQIEEVGAK LRAMMPWIGA NKLVDKAKN
Expression Region 1-339
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Methylocella silvestris Ketol-acid reductoisomerase(ilvC),Baculovirus
Code CSB-BP491148MTF
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDAD INLIKSKKVA IIGYGSQGHA HALNLRDSGV MDVSVALREG SATAAKAAGE GLKVLSVADA AKWADVVMML TPDELQGDIY SEHLAPNLKQ GAALLFAHGL NIHFNLIEPR KDLDVAMVAP KGPGHTVRSE YKRGGGVPCL IAIAQDASGN AHDIALSYAS AIGGGRAGII ETTFREECET DLFGEQVVLC GGLVELIRGG FETLVEAGYA PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VTGPRIVTPE TKAEMKRVLT DIQSGKFTRD WMLENKVNQT SFKATRAKAA SHQIEEVGAK LRAMMPWIGA NKLVDKAKN
Expression Region 1-339
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Methylocella silvestris Ketol-acid reductoisomerase(ilvC),Mammalian cell
Code CSB-MP491148MTF
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDAD INLIKSKKVA IIGYGSQGHA HALNLRDSGV MDVSVALREG SATAAKAAGE GLKVLSVADA AKWADVVMML TPDELQGDIY SEHLAPNLKQ GAALLFAHGL NIHFNLIEPR KDLDVAMVAP KGPGHTVRSE YKRGGGVPCL IAIAQDASGN AHDIALSYAS AIGGGRAGII ETTFREECET DLFGEQVVLC GGLVELIRGG FETLVEAGYA PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VTGPRIVTPE TKAEMKRVLT DIQSGKFTRD WMLENKVNQT SFKATRAKAA SHQIEEVGAK LRAMMPWIGA NKLVDKAKN
Expression Region 1-339
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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