Recombinant Methylocella silvestris Ketol-acid reductoisomerase(ilvC)

Code CSB-YP491148MTF
Size Pls inquire other sizes
Source Yeast
Code CSB-EP491148MTF
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Source E.coli
Code CSB-EP491148MTF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP491148MTF
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Source Baculovirus
Code CSB-MP491148MTF
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names ilvC
Uniprot No. B8EKP4
Species Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Expression Region 1-339
Target Protein Sequence MRVYYDRDAD INLIKSKKVA IIGYGSQGHA HALNLRDSGV MDVSVALREG SATAAKAAGE GLKVLSVADA AKWADVVMML TPDELQGDIY SEHLAPNLKQ GAALLFAHGL NIHFNLIEPR KDLDVAMVAP KGPGHTVRSE YKRGGGVPCL IAIAQDASGN AHDIALSYAS AIGGGRAGII ETTFREECET DLFGEQVVLC GGLVELIRGG FETLVEAGYA PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VTGPRIVTPE TKAEMKRVLT DIQSGKFTRD WMLENKVNQT SFKATRAKAA SHQIEEVGAK LRAMMPWIGA NKLVDKAKN
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
Protein Families Ketol-acid reductoisomerase family
Database Links

KEGG: msl:Msil_3011

STRING: 395965.Msil_3011

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