Recombinant Methylocella silvestris NADH-quinone oxidoreductase subunit B(nuoB)

Product Type Recombinant Protein
Uniprot No. B8EIL8
Species Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Gene Names nuoB
Protein Names Recommended name: NADH-quinone oxidoreductase subunit B EC= 1.6.99.5 Alternative name(s): NADH dehydrogenase I subunit B NDH-1 subunit B
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Subcellular Location Cell inner membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Complex I 20 kDa subunit family
Database Links

KEGG: msl:Msil_2919

STRING: 395965.Msil_2919

Product Details for Different Expression Systems

Product Name Recombinant Methylocella silvestris NADH-quinone oxidoreductase subunit B(nuoB),Yeast
Code CSB-YP491142MTF
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGLTPAPQEG LVINPATDRP LAANDPYFLS INDKLADKGF LVTSTDELIN WARTGSLMWM TFGLACCAVE MMQASMPRYD VERFGFAPRG SPRQSDVMIV AGTLTNKMAP ALRKVYDQMP EPRYVISMGS CANGGGYYHY SYSVVRGCDR IVPVDIYVPG CPPSAEALLY GILLLQRKIR RTGTIER
Expression Region 1-187
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Methylocella silvestris NADH-quinone oxidoreductase subunit B(nuoB),E.coli
Code CSB-EP491142MTF
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGLTPAPQEG LVINPATDRP LAANDPYFLS INDKLADKGF LVTSTDELIN WARTGSLMWM TFGLACCAVE MMQASMPRYD VERFGFAPRG SPRQSDVMIV AGTLTNKMAP ALRKVYDQMP EPRYVISMGS CANGGGYYHY SYSVVRGCDR IVPVDIYVPG CPPSAEALLY GILLLQRKIR RTGTIER
Expression Region 1-187
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Methylocella silvestris NADH-quinone oxidoreductase subunit B(nuoB),Baculovirus
Code CSB-BP491142MTF
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGLTPAPQEG LVINPATDRP LAANDPYFLS INDKLADKGF LVTSTDELIN WARTGSLMWM TFGLACCAVE MMQASMPRYD VERFGFAPRG SPRQSDVMIV AGTLTNKMAP ALRKVYDQMP EPRYVISMGS CANGGGYYHY SYSVVRGCDR IVPVDIYVPG CPPSAEALLY GILLLQRKIR RTGTIER
Expression Region 1-187
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Methylocella silvestris NADH-quinone oxidoreductase subunit B(nuoB),Mammalian cell
Code CSB-MP491142MTF
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MGLTPAPQEG LVINPATDRP LAANDPYFLS INDKLADKGF LVTSTDELIN WARTGSLMWM TFGLACCAVE MMQASMPRYD VERFGFAPRG SPRQSDVMIV AGTLTNKMAP ALRKVYDQMP EPRYVISMGS CANGGGYYHY SYSVVRGCDR IVPVDIYVPG CPPSAEALLY GILLLQRKIR RTGTIER
Expression Region 1-187
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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