Recombinant Human papillomavirus type 16 Minor capsid protein L2 (L2)

Thanks for your inquiry.
① Pls be noted that the Tag of this EP protein is N-terminal 6xHis-SUMO-tagged.
The recombinant proteins are usually used as ELISA standard, in theory, this protein can also be used as the ELISA standard.
However, it is also necessary to see whether the protein matches the antibody in the ELISA. We haven't verified about this for the time being, so we can't 100% guarantee, we recommend you to purchase a small size to have a try first.
② In combination with the your description, we think you want to ask whether the tagged antigen and the untagged antigen have an effect on the affinity of the antibody.
This needs to be determined by combining with the immunogen information of the corresponding antibody and purification method.
a. If the immunogen of your antibody is a fusion protein, purified using proteinA/G or purified by fusion protein affinity chromatography, then choosing untagged antigen as the standard will reduce antibody affinity.
b. If the immunogen of your antibody is a fusion protein, which is purified by untagged target protein affinity chromatography, the antibody affinity is the same for the untagged or tagged/fusion protein.
c. If the immunogen of your antibody is an untagged protein, using proteinA/G purification or antigen affinity chromatography, choosing tagged/fusion protein as the standard will reduce antibody affinity.
Generally, it's better to detect endogenous samples (natural samples). If you want the data to be as accurate as possible, so it is best to use untagged protein for immunization and using untagged protein as the standard.
③ If you need the untagged protein, we can provide tag removal service:
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% successfully.The overall success rate of enzyme digestion data analysis is 75%-86%.
* Not all protein tags can be removed as some proteins will be very unstable after tag removal.
* If we succeed in removing the tag, we will charge for extra cost.
* If we fail in removing the tag, we will provide the fusion protein and won’t charge for any extra cost of tag removal, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
* Generally, the delivery time will be extended for 3 days.
PS: If the tag removal result of the current engineered vector is not good, we will change another vector.