Recombinant Mouse Beta-defensin 2 (Defb2)

Code CSB-YP006705MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP006705MO
MSDS
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Source E.coli
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Code CSB-EP006705MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006705MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006705MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Defb2
Uniprot No.
Alternative Names
Defb2Beta-defensin 2; BD-2; mBD-2; Defensin; beta 2
Species
Mus musculus (Mouse)
Expression Region
21-71
Target Protein Sequence
AVGSLKSIGY EAELDHCHTN GGYCVRAICP PSARRPGSCF PEKNPCCKYM K
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has bactericidal activity.
Gene References into Functions
  1. Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease. PMID: 28622393
  2. NOD2-mediated beta-defensin 2 regulation contributes to the protection against NTHi-induced otitis media PMID: 24625812
  3. mouse urothelium produces mBD2 in response to intravesicular BCG as a defense mechanism against BCG PMID: 25573055
  4. studied the time course of TLR9 and BD-2gene expression by the corneal epithelial cells in herpetic keratitis in C57Bl/6 and BALB/c mice PMID: 22816092
  5. mBD-2 was located in cells with dendritic morphology near mediastinal lymph nodes. mBD-2 may play a role in the control of bacilli growth by contributing to establish a Th1 response, being a link between innate and adaptative immunity. PMID: 22705089
  6. a highly size-specific, TLR4-dependent, innate defense response to fragmented HA contributes to intestinal epithelium barrier defense through the induction of intracellular HbetaD2 protein. PMID: 22761444
  7. Expression and inducibility of mouse beta-defensins-2 was tested in a mouse ocular surface scratch model with and without treatment of supernatants of a clinical Staphylococcus aureus isolate by means of immunohistochemistry. PMID: 20526610
  8. Murine beta-defensins BD2 and BD3 together promote resistance against corneal infection by Pseudomonas aeruginosa. PMID: 19933858
  9. acts directly on immature dendritic cells as an endogenous ligand for Toll-like receptor 4, inducing up-regulation of costimulatory molecules and dendritic cell maturation PMID: 12411706
  10. The results indicate that BD-2 and -3 may be upregulated during the development of autoimmune sialoadenitis. PMID: 17874048
  11. Murine beta-defensin 2 (mDF2beta) can act as an endogenous ligand for TLR-4-activating maturation of dendritic cells. PMID: 18192488
  12. pleural mesothelial cells may contribute to host innate immune defense upon exposure to gram-positive bacteria or their products within the pleural space by upregulating TLR-2 and beta-defensin-2 expression PMID: 18621910
  13. defensin beta 2 production is mediated by TLR2 and TLR4 activation and involves a c-Fos-mediated, protein kinase C-dependent signaling pathway PMID: 18641349
  14. The expression of BD2 was significantly elevated in the lungs of cigarette smoke-exposed mice compared with air-exposed mice. PMID: 18699806
  15. Down regulation of beta-defensin-2 is associated with aggressive tumor development. PMID: 18806743
  16. Murine beta-defensin (mBD)2, but not mBD1, is required for host resistance against Pseudomonas aeruginosa-induced corneal infection. PMID: 19155510

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Subcellular Location
Secreted.
Protein Families
Beta-defensin family
Tissue Specificity
Kidney, uterus and to a lesser extent in heart.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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