Recombinant Mouse Actin, alpha cardiac muscle 1(Actc1)

Code CSB-YP001221MO
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Source Yeast
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Code CSB-EP001221MO
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Source E.coli
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Code CSB-EP001221MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001221MO
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Source Baculovirus
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Code CSB-MP001221MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Actc1
Uniprot No. P68033
Alternative Names Actc1; ActcActin; alpha cardiac muscle 1; Alpha-cardiac actin) [Cleaved into: Actin; alpha cardiac muscle 1; intermediate form]
Species Mus musculus (Mouse)
Expression Region 3-377
Target Protein Sequence DDEETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIS KQEYDEAGPS IVHRKCF
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions
  1. This study provides novel insights into the complex genetic regulation of Actc1 expression in early adult skeletal muscles. PMID: 28847732
  2. Analogous to the findings in human skeletal muscle, Actc1 expression in mouse gastrocnemius muscle increased after testosterone deprivation in orchidectomized mice compared with sham controls PMID: 28756295
  3. ACTC E361G mutation uncouples the relationship between Ca(2+) sensitivity and troponin I phosphorylation, predisposing the heart to dilated cardiomyopathy under conditions of chronic stress. PMID: 26432839
  4. results demonstrate that in ACTC(Co)/KO myofibres, the presence of cardiac alpha-actin instead of skeletal muscle alpha-actin alters actin conformational changes upon activation; this later finely modulates the strain of individual actomyosin interactions and overall lowers myofibre force production PMID: 23784376
  5. Data indicate that the tri-helix bundle is important to the regulatory role of cMyBP-C, likely through actin-binding interactions. PMID: 23782699
  6. propose that the ACTC E99K mutation causes higher myofibrillar Ca(2+) sensitivity that is responsible for the sudden cardiac death, apical hypertrophy, and subsequent development of heart failure in humans and mice. PMID: 21622575
  7. Other actin isoforms cannot compensate for the lack of cardiac alpha-actin, and this seems to induce apoptosis in defective cardiac myocytes, which are not able to cope with the increased workload in the perinatal phase. PMID: 14605248
  8. These results demonstrate the importance of the Emb-MEF2D-histone transacetylase p300 complex in the transcriptional regulation of the cardiac actin gene and suggest a potential role in chromatin remodeling associated with muscle gene activation. PMID: 15024082
  9. there is a cardiac-specific enhancer located within -2.354/-1.36 kbp of Actc1 PMID: 15491989

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Subcellular Location Cytoplasm, cytoskeleton
Protein Families Actin family
Database Links

KEGG: mmu:11464

STRING: 10090.ENSMUSP00000087736

UniGene: Mm.686

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