Recombinant Mouse Acyloxyacyl hydrolase (Aoah), partial

Code CSB-YP001853MO
MSDS
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Source Yeast
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Code CSB-EP001853MO
MSDS
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Source E.coli
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Code CSB-EP001853MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001853MO
MSDS
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Source Baculovirus
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Code CSB-MP001853MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Aoah
Uniprot No.
Alternative Names
AoahAcyloxyacyl hydrolase; EC 3.1.1.77) [Cleaved into: Acyloxyacyl hydrolase small subunit; Acyloxyacyl hydrolase large subunit]
Species
Mus musculus (Mouse)
Expression Region
34-155
Target Protein Sequence
YSHGQSC LGCVVLVSVI EQLAEVHNSS VQVAMERLCS YLPEKLFLKT ACYFLVQTFG SDIIKLLDEA MKADVVCYAL EFCKRGAVQP QCHLYPLPQE AWESALEKAR QVLRRSSTMK YPRSG
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (LPS). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.
Gene References into Functions
  1. Alveolar macrophages increase AOAH expression upon exposure to lipopolysaccharide and that Aoah+/+ mice recover more rapidly than do Aoah-/- mice from acute lung injury induced by nasally instilled lipopolysaccharide or Klebsiella pneumonia. PMID: 28622363
  2. AOAH has a role in modulating microbiota signals that drive Th17 polarization and influence mucosal T-cell immunity PMID: 24344308
  3. role in clearing and catabolizing both free LPS and the LPS in circulating LPS-lipoprotein complexes PMID: 22441700
  4. produced by renal cortical tubule cells and secreted into urine, where it can deacylate lipopolysaccharides, a mechanism to limit inflammatory response to gram-negative bacteria in urinary tract PMID: 15155618
  5. Recovery from the tolerant state induced by Gram-negative bacteria is greatly delayed in in mice that lack AOAH, a lipase that partially deacylates the bacterial cell-wall lipopolysaccharide. PMID: 18779055

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Subcellular Location
Secreted. Cytoplasmic vesicle.
Tissue Specificity
Detected in peritoneal macrophages (at protein level). Strongly expressed in kidney cortex, where it may be produced by proximal tubule cells. In liver, expressed at high levels in Kupffer cells. Expressed by dendritic cells. Detected at low levels in alv
Database Links

KEGG: mmu:27052

UniGene: Mm.314046

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