Recombinant Mouse Ammonium transporter Rh type B (Rhbg), partial

1. Utilizing a structural homology analysis to AmtB, we have identified residues in Rhbg that are implicated in ion transport function. We introduced mutations into these residues and evaluated their function using measurements of intracellular pH (pHi), surface pH (pHs), and whole cell currents (I) that can distinguish between NH3 and NH4+ signals. Our results identified critical amino acids that affect transport. PMID: 27681563
2. Rhbg is critically important in the renal response to metabolic acidosis. in mice with intact Rhbg expression, metabolic acidosis does not induce maximal changes in PEPCK, NHE-3, and GS expression despite the presence of persistent metabolic acidosis. PMID: 24338819
3. It was concluded that hypokalemia increases Rhbg expression in intercalated cells in the cortex and outer medulla and that intercalated cell Rhbg expression is necessary for the normal increase in renal ammonia excretion in response to hypokalemia. PMID: 23220726
4. These data indicate that transport of NH(4)(+) and methyl ammonium (+) by Rhbg is pH sensitive. PMID: 20810915
5. Rhbg in connecting segment and principal cells may contribute to renal ammonia excretion PMID: 20719974
6. Basolateral RhBG is expressed by perivenous hepatocytes, where it may mediate ammonium uptake PMID: 12730882
7. mIMCD-3 cells express a basolateral electroneutral NH4+/H+ exchange activity that may be mediated by RhBG. PMID: 15149971
8. Characterization of the role of Rhbg in ammonium ion transport using voltage clamp techniques. PMID: 15353405
9. concluded that Rhbg is not a critical determinant of NH(4)(+) excretion by the kidney and of NH(4)(+) detoxification by the liver in vivo PMID: 16077082
10. RhBG-mediated transport is an electroneutral process that is driven by the NH4+ concentration and the transmembrane H+ gradient, effectively exchanging NH4+ for H+ in a process that results in transport of net NH3. PMID: 16563829
11. Review. Animal studies show Rhbg has a role in transmembrane NH3 or NH4+ transport in epithelial cells. The absence of distal tubular acidosis or hyperammonemia in Rhbg KO mice raises new questions about the physiological significance of this protein. PMID: 16563831
12. Review. Rhbg is in distal nephron sites responsible for NH3 secretion and in perivenous hepatocyte basolateral plasma membrane. Cetabolic acidosis does not affect Rhbg, so it may be either an ammonia- sensor or mediate other roles. PMID: 16564723
13. Rhbg transport of NH4+ is electrogenic. Methylammonium is transported by Rhbg. NH4+ transport by Rhbg is saturated at high concentrations with Michaelis-Menten kinetics. PMID: 16580864

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KEGG: mmu:58176

STRING: 10090.ENSMUSP00000130767

UniGene: Mm.103777