Recombinant Mouse Band 4.1-like protein 5 (Epb41l5)

Code CSB-YP806360MO
MSDS
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Source Yeast
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Code CSB-EP806360MO
MSDS
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Source E.coli
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Code CSB-EP806360MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806360MO
MSDS
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Source Baculovirus
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Code CSB-MP806360MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Epb41l5
Uniprot No.
Alternative Names
Epb41l5; Epb4.1l5; Kiaa1548; Band 4.1-like protein 5
Species
Mus musculus (Mouse)
Expression Region
1-731
Target Protein Sequence
MLSFLRRTLG RRSMRKHAEK ERLREAQRAA THIPAAGDAK SIITCRVSLL DGTDVSVDLP KKAKGQELFD QIMYHLDLIE SDYFGLRFMD SAQVAHWLDG TKSIKKQVKI GSPYCLHLRV KFYSSEPNNL REELTRYLFV LQLKQDILSG KLECPFDTAV QLAAYNLQAE LGDYDLAEHS PELVSEFRFV PIQTEEMELA IFEKWKEYRG QTPAQAETNY LNKAKWLEMY GVDMHVVKAR DGNDYSLGLT PTGVLVFEGE TKIGLFFWPK ITRLDFKKNK LTLVVVEDDD QGKEQEHTFV FRLDHPKACK HLWKCAVEHH AFFRLRGPVQ KSSHRSGFIR LGSRFRYSGK TEYQTTKTNK ARRSTSFERR PSKRYSRRTL QMKASTTQPE DLGVLNASAQ KSDSQQAWGV MSPVPVTSSS SCGAVQVEIE NLPQTSATEQ HDRKCLPLSV DLLNSPDLLE TTIGDVTRTS ETSAPFPAPD TINVATRSNE LEEFKAECET LKDDTEKLKQ LETEQTILPS LRPTIDINVN SQEEVVKLTE KCLNNAIENP ALNAVKVPPD FKSNILKAQV EAVHKVTRED SLLTHKNASV QDAATNSTAF NENDVPVCKD SLTPVHGTAA DSASVLKDAT DELDALLLSL TENLMDHTVT PQVSSPSMIT PRWIIPQSAT ISNGLAGYGA SLAGTDECSQ KDGFSLISPP APFLVDAVTS SAPPLPEDST LKQKCLLTTE L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells.
Gene References into Functions
  1. Genetic deletion of Epb41l5 resulted in severe proteinuria, detachment of podocytes, and development of focal segmental glomerulosclerosis. Remarkably, by binding and recruiting the RhoGEF ARGHEF18 to the leading edge, EPB41L5 directly controls actomyosin contractility and subsequent maturation of focal adhesions, cell spreading, and migration. PMID: 28536193
  2. Left-right patterning in the mouse requires Epb4.1l5-dependent morphogenesis of the node and midline PMID: 20678497
  3. Lulu mutation in Epb4.1l5 helps anchor the actin-myosin contractile machinery to the membrane to allow the dynamic rearrangements of epithelia that mediate embryonic morphogenesis. PMID: 17507402
  4. the importance of a conserved Crumbs-MPP5-EPB41L5 polarity complex in mammals for separation of the apical and basolateral domains through specialized cell-cell junctions PMID: 17920587
  5. Results describe the involvement of EPB41L5 in epithelial-mesenchymal transition during mouse gastrulation. PMID: 18794329

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Subcellular Location
Cytoplasm. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein. Photoreceptor inner segment.
Tissue Specificity
In the retina, expressed at the outer limiting membrane, retinal pigment epithelium, outer nuclear layer and outer plexiform layer (at protein level). Also detected in the inner segments (at protein level). Expressed at the basolateral and apical sides of
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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